F23208

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23208

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23208

Determination of introduced cDNA(s)

  • RAFL09-50-P13 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-50-P13 - Resource: pda06471

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-50-P13 - full reading 
    AAAAGAAGCA GTTAGATTGT GACGGAGGAG ACAAAATCAT CATCTCTGTT 
    CTTCTTCTTC TCCTCTCTCA CATACGATGA TGACCCAACA ACCTTGACGT 
    TGATTTCGAT GTCACACACA ACACTATAAA TAACACTCTT CACCTATTTT 
    TGTCATCTCA CACTTTGGAC TTTGGAGCCC AAAATAATCT CATAACCATT 
    AAAAACAAAT CTCCTCCTTC TCTCCGGCGT CAATGGAGGA TCTCCGTCGT 
    AGATTTCCGA CTAAAAAGAA CGGAGAAGAA ATCTCTAATG TCGCCGTCGA 
    TCCTCCTCTT CGTAAAGCCT CTGACGCGCT TCCTCTTCCG TTATACCTAA 
    CCAACACTTT TTTCCTCTCG CTCTTCTTCG CTACTGTTTA TTTTCTTCTT 
    AGCCGATGGC GTGAGAAGAT CCGTAACTCG ACGCCTCTTC ACGTCGTTGA 
    CTTATCTGAG ATCTGTGCTC TAATCGGTTT CGTTGCTTCG TTTATCTACC 
    TCCTTGGTTT CTGTGGTATT GATCTCATTT TCCGATCTTC TTCCGATGAT 
    GATGTTTGGG TTAACGATGG GATGATTCCA TGTAATCAAT CCCTAGATTG 
    TCGTGAGGTT CTTCCGATTA AACCTAATTC CGTCGATCCT CCTCGGGAAT 
    CGGAGCTTGA TTCGGTGGAA GATGAAGAAA TCGTTAAATT AGTGATCGAC 
    GGAACCATCC CTTCGTACTC ATTGGAGACG AAACTCGGAG ATTGTAAAAG 
    AGCAGCAGCG ATTAGAAGAG AAGCGGTTCA GAGGATAACC GGTAAATCCT 
    TAACCGGTCT TCCATTGGAA GGTTTCGATT ACAATTCAAT TTTGGGGCAA 
    TGCTGTGAGA TGCCAGTTGG GTATGTTCAG ATCCCAGTGG GGATTGCAGG 
    ACCTTTGTTG CTTGATGGGG TTGAGTATTC AGTGCCAATG GCTACAACAG 
    AAGGTTGTTT GGTTGCTAGT ACCAATAGAG GTTTTAAAGC TATACACTTG 
    TCTGGTGGTG CTTTTAGTGT TCTTGTGAAA GATGCCATGA CTAGAGCTCC 
    AGTTGTTAGA TTCCCTTCTG CAAGAAGAGC TGCTCTTGTC ATGTTTTATC 
    TTCAAGATCC TTCTAATTTC GAGAGATTGT CTCTCATTTT CAACAAATCG 
    AGTAGATTTG CTAGGCTTCA GAGTATTACA TGCACGATTG CTGGGAGGAA 
    TCTGTATCCG AGGTTTGCGT GTAGTACTGG TGATGCTATG GGGATGAACA 
    TGGTCTCAAA AGGTGTTCAG AATGTCTTGG ACTTTGTAAA GAGCGAGTTT 
    CCTGACATGG ATGTTATTGG CATCTCTGGG AACTACTGTT CGGACAAGAA 
    GGCTTCGGCT GTAAACTGGA TCGAAGGACG TGGTAAGCAT GTTGTATGTG 
    AAGCTTTTAT TAAGGCTGAG ATTGTGGAGA AAGTGTTGAA GACTAGCGTT 
    GAGGCTCTTG TAGAGCTTAA CACGCTCAAG AATCTTGTTG GTTCAGCCAT 
    GGCGGGTTCT CTTGGTGGGT TCAATGCTCA TTCAAGCAAT ATTGTCAGCG 
    CTGTGTTCAT CGCCACTGGC CAAGACCCAG CTCAAAACGT GGAGAGCTCA 
    CACTGTATGA CCATGATTCT TCCCGATGGT GACGACCTTC ACATCTCTGT 
    TTCAATGCCT TGCATTGAGG TTGGTACTGT TGGAGGTGGG ACACAACTTG 
    CATCACAAGC AGCTTGTTTG AATCTACTCG GTGTGAAAGG ATCAAACAAC 
    GAGAAACCTG GCTCGAACGC ACAGCAATTG GCAAGAATAG TGGCTGGTTC 
    GGTTCTTGCA GGAGAGCTTT CACTAATGTC TGCTATTGCA GCTGGACAGC 
    TTGTGAAGAG TCACATGAAA TACAACAGAT CCAGCAGAGA CATTGGCCCT 
    TCGTCTCAAG TCAACAGGTG ATGTTTGGAG CACGATGAGA CGGATAATAA 
    TAAAACAACA AAAACCCATA ATTAGATTTG ATGTAATTCT AACTTCATTT 
    GATTTCTTCT TCATCGATCT CTTTTCTTTT CTATCTATTT TGTACTAAGT 
    CTCCAAATTT TCTTAATTTA TGTAAAGTCT TTTGTCCTTC G

    Gene models with high sequence identity

    1. AT2G17370.1 [+] show detail - 3-hydroxy-3-methylglutaryl-CoA reductase 2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Hydroxymethylglutaryl-CoA reductase, class I, catalytic 2.3e-203
    Gene3D Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 9.8e-83
    FPrintScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 1.1e-83
    FPrintScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 1.1e-83
    FPrintScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 1.1e-83
    FPrintScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 1.1e-83
    FPrintScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 1.1e-83
    FPrintScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 1.1e-83
    superfamily Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding 8.7e-101
    superfamily Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding 4e-55
    HMMPfam Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 8.8e-158
    ProfileScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 135.581
    HMMPanther Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 0
    PatternScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 0
    PatternScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 0
    PatternScan Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic 0

    See the detailed result >

    AT2G17370.1

    Model type
    Protein coding
    Short Description
    3-hydroxy-3-methylglutaryl-CoA reductase 2
    Curator Summary
    Encodes a 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) that is involved in the synthesis of sterol and triterpenoid compounds.
    Computational Description
    3-hydroxy-3-methylglutaryl-CoA reductase 2 (HMG2); CONTAINS InterPro DOMAIN/s: Hydroxymethylglutaryl-CoA reductase, class I, catalytic (InterPro:IPR004554), Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding (InterPro:IPR009029), Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding (InterPro:IPR009023), Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic (InterPro:IPR002202); BEST Arabidopsis thaliana protein match is: hydroxy methylglutaryl CoA reductase 1 (TAIR:AT1G76490.1); Has 2106 Blast hits to 2105 proteins in 886 species: Archae - 203; Bacteria - 937; Metazoa - 224; Fungi - 225; Plants - 261; Viruses - 1; Other Eukaryotes - 255 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR