F23315

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23315

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23315

Determination of introduced cDNA(s)

  • RAFL05-11-M07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-11-M07 - Resource: pda02519

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-11-M07 - full reading 
    ATAAATGCTC TTCTCCTCCG ATCTCTCCGC CATAAAATCT ACTCTTTCTT 
    CTTCTGGGTG TTCAAAGAAT TAGTCGTTCT CGCGTATTCA CTTTCCATGG 
    CGGGCTTTCT CTCCGTTGTC CGGCGCGTGT ACCTCACTCT CTACAATTGG 
    ATCGTCTTTG CTGGATGGGC TCAGGTTTTG TACTTAGCGA TAACGACGTT 
    GAAGGAAACG GGTTATGAGA ATGTCTATGA CGCCATTGAG AAGCCTCTCC 
    AGCTTGCTCA GACTGCCGCC GTTCTCGAGA TTCTTCATGG ATTAGTAGGT 
    TTGGTCAGAT CTCCTGTTTC TGCAACTCTG CCACAGATAG GTTCAAGGCT 
    ATTTCTCACT TGGGGCATCC TATACAGTTT TCCAGAGGTC CGATCTCATT 
    TTCTTGTTAC GTCGCTGGTC ATAAGCTGGT CTATCACAGA GATTATTCGC 
    TACTCATTCT TCGGCTTTAA GGAAGCTCTA GGCTTTGCAC CTTCATGGCA 
    CTTGTGGCTC AGATACAGCA GCTTTTTATT GCTATACCCT ACCGGTATCA 
    CCAGCGAAGT TGGTCTTATC TACCTTGCCT TACCACACAT CAAGACATCG 
    GAGATGTACA GCGTTAGGAT GCCTAACATA TTGAACTTCT CATTTGACTT 
    CTTCTATGCA ACGATACTCG TCCTTGCAAT CTATGTCCCA GGTAGCCCAC 
    ACATGTACAG GTACATGCTT GGTCAGCGAA AGAGAGCTCT CTCCAAATCC 
    AAGAGGGAAT AAAAGAAAAG ATTTAGAAAG AGACGAAAAA GCTCATCAAC 
    TGTTTTCTCC TCTTGTCTTG TTCGTTCATG TACTACTAGT CTACTACTAC 
    AACTTGTCTT TTGTTTAGCT TGTTTGAGCT TTTACTTCCT CGGCGATTAC 
    TTGAACTTAC ATGCAGCAGG ATACTATTAA GTGTAATGTG GTTTCTAAAT 
    TAATAAGAAA ATCATTGGTT CCTT

    Gene models with high sequence identity

    1. AT5G10480.1 [+] show detail - Protein-tyrosine phosphatase-like, PTPLA
      E-value: 0; Score: 99.90

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Protein-tyrosine phosphatase-like, PTPLA 4.1e-60
    HMMPanther Protein-tyrosine phosphatase-like, PTPLA 1.9e-87

    See the detailed result >

    AT5G10480.1

    Model type
    Protein coding
    Short Description
    Protein-tyrosine phosphatase-like, PTPLA
    Curator Summary
    Protein tyrosine phosphatase-like involved in cell division and differentiation. Interacts with CDKA;1 only in its phosphorylated form, preventing dephosphorylation. Overexpression slowed down cell division in suspension cell cultures at the G2-to-M transition and early mitosis and inhibited Arabidopsis seedling growth. Localized in the cytoplasm of dividing cells but moved into the nucleus upon cell differentiation. Based on complementation of yeast mutant PAS2 has acyl-CoA dehydratase activity. It interacts with CER10, a component of the microsomal fatty acid elongase complex, suggesting a role in synthesis of VLCFAs (very long chain fatty acids).
    Computational Description
    PASTICCINO 2 (PAS2); CONTAINS InterPro DOMAIN/s: Protein-tyrosine phosphatase-like, PTPLA (InterPro:IPR007482); BEST Arabidopsis thaliana protein match is: Protein-tyrosine phosphatase-like, PTPLA (TAIR:AT5G59770.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR