F23404

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23404

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
stem (PO:0009047)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
T2  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23404

Determination of introduced cDNA(s)

  • RAFL05-07-F16 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-07-F16 - Resource: pda01191

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-07-F16 - full reading
    ACTCTCAAAA TCATCTTCTT CACCCAAAAA CCCCTAAAAG CCTTATCCCT 
    TCTTCTTCCC ATGGCTGCTT CAGCTTCGTC TCTCGCTCTC TCTTCCTTCA
    ACCCTAAATC TCTTCCTTTC GGCGTCTCCA GACCTGCCTC CGTTTCCCTC
    TTATCTCCTT CCCTCTCCTT TAAACTCAAT TCCGACTCCG TTTCCTTCTC
    CATCGCCGCC AAATGGAACT CTCCCGCTTC TCGCTTCGCC CGTAACGTTG
    CGATTACCTC AGAGTTCGAG GTGGAAGAAG ATGGTTTCGC TGACGTCGCT
    CCGCCAAAAG AGCAATCTTT CTCTGCTGAC CTTAAACTCT TCGTTGGTAA
    CCTTCCTTTC AACGTTGACA GTGCTCAGCT CGCTCAGCTC TTTGAGAGTG
    CCGGAAATGT TGAGATGGTT GAGGTAATCT ATGACAAAAT TACAGGAAGA
    AGCAGAGGTT TTGGATTCGT GACTATGTCT TCAGTTTCTG AAGTTGAGGC
    AGCTGCTCAG CAGTTCAATG GCTATGAGTT GGATGGTAGA CCTTTGAGAG
    TCAATGCTGG TCCCCCACCA CCAAAGAGGG AAGATGGTTT CTCCAGAGGA
    CCTAGGAGCA GCTTTGGAAG CTCAGGTTCT GGATATGGAG GAGGTGGTGG
    TTCTGGTGCT GGTTCAGGAA ACCGTGTTTA TGTGGGTAAC CTCTCTTGGG
    GAGTTGATGA CATGGCTCTT GAGAGTTTGT TCTCGGAGCA AGGAAAGGTT
    GTTGAGGCCA GAGTCATCTA CGACAGGGAC AGTGGTCGAT CCAAGGGTTT
    TGGATTTGTG ACATACGACT CTTCTCAAGA GGTCCAAAAT GCCATCAAGT
    CCTTGGATGG TGCTGATTTG GACGGTAGAC AAATTAGAGT CTCGGAAGCT
    GAGGCTAGGC CTCCAAGGCG CCAATATTGA GCACCAATCT ATGACTTCCT
    ATTCTCAAAA ACGCATATTC TGGAGGGCGC TTCGAAGTAA AGAGGGTTTG
    TGAGATGATG GCAGTTTCAG ACGGTACTAA GCTCTTAGCT TCGCCTATGT
    TTGTTGCCTT GGATGCAAGA AGGTCGTAAA GGAATGGTCT TTTTTTTTTT
    GAGAAACGTA TAATTAAGAT AGAAACTGGA GAGACCATGT TCTTGTCTGT
    CTGAATGCTG CCATTGACTG CTTCGCTTTG GTTTTGATTC AATTTTTTTT
    TCTCTCTATA TCGTGGTCTC TTCTTCCTTA AAAAAAAAAA AAAAA

    Gene models with high sequence identity

    1. AT2G37220.1 [+] show detail - RNA-binding (RRM/RBD/RNP motifs) family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart RNA recognition motif, RNP-1 5.7e-28
    HMMSmart RNA recognition motif, RNP-1 1.8e-28
    HMMPfam RNA recognition motif, RNP-1 1.7e-20
    HMMPfam RNA recognition motif, RNP-1 1.2e-21
    HMMPanther RIBONUCLEOPROTEIN, CHLOROPLAST 1.2e-80
    HMMPanther RNA-BINDING PROTEIN 1.2e-80
    Gene3D Nucleotide-binding, alpha-beta plait 3.7e-31
    Gene3D Nucleotide-binding, alpha-beta plait 5.4e-27
    ProfileScan RNA recognition motif, RNP-1 19.041
    ProfileScan RNA recognition motif, RNP-1 20.525
    superfamily RNA-binding domain, RBD 8.2e-28
    superfamily RNA-binding domain, RBD 5.7e-26

    See the detailed result >

    AT2G37220.1

    Model type
    Protein coding
    Short Description
    RNA-binding (RRM/RBD/RNP motifs) family protein
    Curator Summary
    Encodes a chloroplast RNA binding protein. A substrate of the type III effector HopU1 (mono-ADP-ribosyltransferase). Protein is tyrosine-phosphorylated and its phosphorylation state is modulated in response to ABA in Arabidopsis thaliana seeds.
    Computational Description
    RNA-binding (RRM/RBD/RNP motifs) family protein; FUNCTIONS IN: RNA binding, poly(U) RNA binding; INVOLVED IN: response to cold, response to abscisic acid stimulus, innate immune response; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: chloroplast RNA-binding protein 29 (TAIR:AT3G53460.3); Has 997478 Blast hits to 497195 proteins in 22066 species: Archae - 21542; Bacteria - 603238; Metazoa - 188798; Fungi - 28200; Plants - 60491; Viruses - 70820; Other Eukaryotes - 24389 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR