F23643

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23643

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23643

Determination of introduced cDNA(s)

  • RAFL06-08-N17 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-08-N17 - Resource: pda17162

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-08-N17 - full reading 
    GATTCCACCA GTCTCTCGCA ATCTTCGCTC TTCTCTTTGC TCTCTCTCTC 
    TCAACGCGGT TCAGATCCGA GTTTGGGAGA TTCAAGCTCC CTGAAAAAAG 
    CCCTTTACTC TCGCTTACCA ACTTTGCTCC AGCATGGCAG GATCTGCACC 
    AGAAGGCACA CAGTTTGATG CACGTCAGTT TGACCAGAAA CTCAATGAAG 
    TACTTGAGGG ACAAGATGAG TTCTTCACAT CCTATGATGA TGTTCATGAG 
    AGCTTTGATG CCATGGGTCT TCAAGAGAAC CTTCTCAGGG GTATTTATGC 
    TTATGGTTTT GAGAAGCCTT CTGCTATTCA GCAAAGAGGA ATCGTCCCCT 
    TCTGTAAGGG TCTTGATGTG ATTCAACAGG CTCAGTCTGG TACTGGGAAA 
    ACAACAACTT TCTGCTCTGG TGTCTTGCAG CAGCTGGACT TCTCCCTTAT 
    CCAGTGTCAG GCTTTGGTTC TAGCTCCAAC TAGAGAGCTT GCTCAGCAAA 
    TTGAGAAGGT CATGAGGGCC CTTGGTGATT ACCTTGGTGT CAAGGTTCAC 
    GCCTGTGTTG GTGGAACCAG TGTCCGTGAG GATCAGCGCA TCCTCCAAGC 
    TAGTGTCCAT GTTGTTGTTG GAACTCCAGG GCGTGTCTTT GACATGTTGA 
    AGAGGCAGTC TCTTCGTGCT GACAACATCA AGATGTTTGT TCTCGATGAA 
    GCTGATGAAA TGCTCTCCCG TGGTTTCAAG GACCAGATCT ATGACATATT 
    CCAGCTTCTT CCACCAAAGA TCCAAGTTGG TGTGTTCTCC GCAACAATGC 
    CACCAGAAGC TCTTGAGATC ACAAGGAAGT TCATGAGCAA GCCAGTGAGA 
    ATCTTGGTGA AGCGTGATGA GCTTACCCTT GAAGGTATCA AGCAGTTCTA 
    CGTCAACGTT GAGAAAGAAG AGTGGAAGCT CGAGACACTC TGTGATCTCT 
    ACGAGACTCT TGCCATCACT CAGAGTGTCA TCTTCGTGAA CACCAGGCGT 
    AAGGTTGATT GGCTCACAGA CAAAATGAGA AGCCGTGACC ACACAGTCTC 
    TGCAACTCAC GGAGACATGG ACCAGAACAC CAGGGACATC ATCATGAGAG 
    AGTTCAGGTC TGGTTCCTCC CGTGTTCTCA TCACCACTGA CCTCTTAGCT 
    CGTGGTATTG ATGTCCAACA AGTCTCCCTG GTTATCAACT TCGATCTCCC 
    AACTCAGCCA GAGAACTACC TTCACCGTAT CGGAAGAAGT GGAAGGTTCG 
    GGAGAAAGGG TGTTGCGATC AATTTCGTGA CCCGTGATGA TGAGAGGATG 
    CTGTTTGATA TTCAGAAATT CTACAATGTG GTTGTCGAAG AGCTGCCTTC 
    GAACGTGGCC GATCTGCTGT GAAGGGAGAA AAGAAGTCGG TTCTGTTGCT 
    GTGTTTGCTC TGCCGTACTA GTATCACCGG AGAAGAAAGG TTTTCTTGTT 
    TTGCAAGGCA CTCTTTGATC TGATTTTGAC CCGTCGTCTA TCAGCGATTT 
    ACATTTCTTA AATTTTCTTA GTTGTTGTTC TCTCCTCTCT CTCCCAGCTA 
    CGAACATATC TCTCTCTTCT TGACCATTTT TAAGTTTAAG TCATATGGAG 
    TATTTATTTC TTCAAAAAAA AGA

    Gene models with high sequence identity

    1. AT3G13920.1 [+] show detail - eukaryotic translation initiation factor 4A1
      E-value: 0; Score: 99.88

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam DNA/RNA helicase, DEAD/DEAH box type, N-terminal 1.1e-43
    HMMPfam DNA/RNA helicase, C-terminal 1.4e-25
    ProfileScan Helicase, superfamily 1/2, ATP-binding domain 29.566
    ProfileScan DNA/RNA helicase, C-terminal 25.831
    ProfileScan RNA helicase, DEAD-box type, Q motif 10.433
    Gene3D no description 7.6e-68
    Gene3D no description 5.6e-51
    PatternScan RNA helicase, ATP-dependent, DEAD-box, conserved site 0
    HMMSmart DEAD-like helicase, N-terminal 3.7e-56
    HMMSmart DNA/RNA helicase, C-terminal 2.9e-31
    superfamily P-loop containing nucleoside triphosphate hyd 1.3e-56
    superfamily P-loop containing nucleoside triphosphate hyd 8e-48
    HMMPanther EUKARYOTIC INITIATION FACTOR 4A 1.1e-300
    HMMPanther DEAD BOX ATP-DEPENDENT RNA HELICASE 1.1e-300

    See the detailed result >

    AT3G13920.1

    Model type
    Protein coding
    Short Description
    eukaryotic translation initiation factor 4A1
    Curator Summary
    eukaryotic translation initiation factor 4A-1
    Computational Description
    eukaryotic translation initiation factor 4A1 (EIF4A1); FUNCTIONS IN: protein binding, ATP-dependent helicase activity, translation initiation factor activity; INVOLVED IN: response to cadmium ion, translational initiation; LOCATED IN: cytosol, nucleolus, cell wall, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: RNA helicase, DEAD-box type, Q motif (InterPro:IPR014014), DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), RNA helicase, ATP-dependent, DEAD-box, conserved site (InterPro:IPR000629), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: eif4a-2 (TAIR:AT1G54270.1); Has 49714 Blast hits to 49117 proteins in 3142 species: Archae - 779; Bacteria - 27442; Metazoa - 6117; Fungi - 4773; Plants - 2624; Viruses - 17; Other Eukaryotes - 7962 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR