F25010

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F25010

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F25010

Determination of introduced cDNA(s)

  • RAFL05-18-A09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-18-A09 - Resource: pda02746

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-18-A09 - full reading
    CTCGGAGATT CAATCACTCA TGGCGAACGA AGACGACGAC GGTGAGAAAT 
    CCAGAAGTCT GCACCAACAG ATTGCTAGAA AACCCAAAAC CCAGATCGTG
    ATTGGTGTGC CATCGTACCA GGAAGTTTTG GAAAGCTCAC AGACGAAGTC
    CACGCCTCCA TCTCTCTTCA AGCCTTCCCA GAGTTTCTCT CAGGCTTTCG
    CTTTCGTCAA GTCGTCCGAC GTCTACTCTC CTCCTCCTCC TTCCTCCGCA
    GCCGCCTCTT CTTCTCAGCC GTCGGGCGCT TCTCAGGTGC CCCACAGTTC
    AAGCCAAACG CATCAAACTG ATGGAGCCTC ATCTTCTTCT ACTCCTGTAG
    CCACTGGCTC TGTACCATCA AACACAACTC AAAATCGTAA CGCTATTCTC
    GTTAGCCATC GACAGAAAGG AAACCCGCTT CTTAAACATA TCAGGAATGT
    GAAATGGGTT TTCTCAGATA TCATTCCAGA TTACGTGCTT GGTCAAAACT
    CTTGCGCTTT GTATTTAAGC TTGCGGTATC ATCTCCTACA TCCGGATTAC
    CTCTATTTCC GCATTAGGGA ACTACAAAAG AATTTCAAGC TAAGTGTTGT
    TCTTTGCCAT GTCGACGTGG AAGATACGGT GAAACCGTTG CTTGAAGTCA
    CAAAAACCGC TCTACTTCAT GATTGCACTC TATTGTGTGC CTGGAGCATG
    ACAGAATGTG CCCGTTATTT GGAAACGATA AAAGTCTATG AAAACAAACC
    TGCAGATCTC ATCCAAGGCC AAATGGATAC AGATTACCTT TCACGGCTAA
    ACCATTCTCT TACAAGCATT CGACATGTAA ACAAGAGCGA TGTAGTTACA
    CTTGGTTCTA CATTCGGGTC TCTTGCTCAT ATAATCGATG CATCCATGGA
    AGATTTAGCA CGTTGTCCCG GGATTGGCGA ACGTAAGGTG AAGCGGTTGT
    ATGATACATT CCACGAGCCT TTCAAGCGTG CAACTTCGAG CTACCCGTCT
    GTTGTAGAAC CACCAATCCC GGAAGCTCCA GTTGAGAAGG ATGTGAACTC
    AGAGGAACCT GTTGAAGAAG ACGAGGATTT TGTGGAGGAC TCGAGAAAAC
    GCAAGAAGAA GGAGCCCGAG CCCGAGAAAA CTGTGAAGAC CGCACTCTCT
    GCTGTTTTTG CTCGTTACTC TGATAGACTT AGCAAGAAAA AGGAGAAACA
    GAAGGAGAAA GATACAACAA CGGCGTCAGA TGCTGAAACC CACCAAAATT
    AACACGGTTT TGTTCCTTGG GTAAGGCCAT GGTGACCACA TTCCTAGGAT
    TGTAACTTAA TGATAATAAA AAAGCTTTAC TGTGTCTAAT TTGTGTGATC
    TCTACCATAG AATTGGTTGG TTATTGAAGC CTGCGTGTAT AAATTTCAAA
    GGGTTGTTAC TACTTTGTTA TCTTTTTTCT TGGTCTGGGC CATGTGCAAT
    AGTATTATAA ACAAACAAAA C

    Gene models with high sequence identity

    1. AT3G05210.1 [+] show detail - nucleotide repair protein, putative
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily RuvA domain 2-like 5e-16
    HMMPfam DNA repair protein rad10 1.4e-29
    BlastProDom DNA repair protein rad10 1e-69
    Gene3D no description 8.4e-05
    HMMPanther EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1 3.6e-113
    HMMTigr DNA repair protein rad10 5.7e-53

    See the detailed result >

    AT3G05210.1

    Model type
    Protein coding
    Short Description
    nucleotide repair protein, putative
    Curator Summary
    encodes a homolog of human ERCC1 protein (yeast RAD10), which is a DNA repair endonuclease. Mutants are sensitive to UV-B and gamma radiation (G2 cell cycle phase arrest) and are defective in dark-repair of pyrimidine pyrimidone dimers. This protein incises the 5' end of damaged DNA, similar to ERCC1/RAD10.
    Computational Description
    ERCC1; FUNCTIONS IN: 5'-flap endonuclease activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Helix-hairpin-helix DNA-binding motif, class 1 (InterPro:IPR003583), DNA repair protein rad10 (InterPro:IPR004579), RuvA domain 2-like (InterPro:IPR010994); Has 658 Blast hits to 452 proteins in 188 species: Archae - 0; Bacteria - 4; Metazoa - 166; Fungi - 153; Plants - 44; Viruses - 0; Other Eukaryotes - 291 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR