F25020

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F25020

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
stem (PO:0009047)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F25020

Determination of introduced cDNA(s)

  • RAFL04-19-J21 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-19-J21 - Resource: pda00667

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-19-J21 - full reading 
    ATCATCATCT TCTTCTTCAA TGGCCTTCAC CGCTTTTCTC CTCCTCCTCT 
    CTTCCATCGC CGCCGGCTTC CTCCTCCTAC TCCGCCGTAC ACGTTACCGT 
    CGGATGGGTC TGCCTCCGGG AAGCCTTGGT CTCCCTCTGA TAGGAGAGAC 
    TTTTCAGCTG ATCGGAGCTT ACAAAACAGA GAACCCTGAG CCTTTCATCG 
    ACGAGAGAGT AGCCCGGTAC GGTTCGGTTT TCATGACGCA TCTTTTTGGT 
    GAACCGACGA TTTTCTCAGC TGACCCGGAA ACGAACCGGT TTGTTCTTCA 
    GAACGAAGGG AAGCTTTTTG AGTGTTCTTA TCCTGCTTCC ATTTGTAACC 
    TTTTGGGGAA ACACTCTCTG CTTCTTATGA AAGGTTCTTT GCATAAACGT 
    ATGCACTCTC TCACCATGAG CTTTGCTAAT TCTTCAATCA TTAAAGACCA 
    TCTCATGCTT GATATTGACC GGTTAGTCCG GTTTAATCTT GATTCTTGGT 
    CTTCTCGTGT TCTCCTCATG GAAGAAGCCA AAAAGATAAC GTTTGAGCTA 
    ACGGTGAAGC AGTTGATGAG CTTTGATCCA GGGGAATGGA GTGAGAGTTT 
    AAGGAAAGAG TATCTTCTTG TCATCGAAGG CTTCTTCTCT CTTCCTCTCC 
    CTCTCTTCTC CACCACTTAC CGCAAAGCCA TCCAAGCGCG GAGGAAGGTG 
    GCGGAGGCGT TGACGGTGGT GGTGATGAAA AGGAGGGAGG AGGAGGAAGA 
    AGGAGCGGAG AGAAAGAAAG ATATGCTTGC GGCGTTGCTT GCGGCGGATG 
    ATGGATTTTC CGATGAAGAG ATTGTTGACT TCTTGGTGGC TTTACTTGTC 
    GCCGGTTATG AAACAACCTC CACGATCATG ACTCTCGCCG TCAAATTTCT 
    CACCGAGACT CCTTTAGCTC TTGCTCAACT CAAGGAAGAG CATGAAAAGA 
    TTAGGGCAAT GAAGAGTGAT TCGTATAGTC TTGAATGGAG TGATTACAAG 
    TCAATGCCAT TCACACAATG TGTGGTTAAT GAGACGCTAC GAGTGGCTAA 
    CATCATCGGC GGTGTTTTCA GACGTGCAAT GACGGATGTT GAGATCAAAG 
    GTTATAAAAT TCCAAAAGGG TGGAAAGTAT TCTCATCGTT TAGAGCGGTT 
    CATTTAGACC CAAACCACTT CAAAGATGCT CGCACTTTCA ACCCTTGGAG 
    ATGGCAGAGC AACTCGGTAA CGACAGGCCC TTCTAATGTG TTCACACCGT 
    TTGGTGGAGG GCCAAGGCTA TGTCCCGGTT ACGAGCTGGC TAGGGTTGCA 
    CTCTCTGTTT TCCTTCACCG CCTAGTGACA GGCTTCAGTT GGGTTCCTGC 
    AGAGCAAGAC AAGCTGGTTT TCTTTCCAAC TACAAGAACG CAGAAACGGT 
    ACCCGATCTT CGTGAAGCGC CGTGATTTTG CTACTTGAAG AAGAAGAGAC 
    CCATCTGATT TTATTTATAG AACAACAGTA TTTTTCAGGA TTAATTTCTT 
    CTTCTTTTTT TGCCTCCTTG TGGGTCTAGT GTTTGACAAT AAAAGTTATC 
    ATTACTCTAT AAAGCCTTAG CTTCTGTGTA CATAAAAAAA AAAAACTTTT 
    GTTTACCTTA TGCTTGCATA AATCTCTTCT GCTTCAATGG T

    Gene models with high sequence identity

    1. AT5G05690.1 [+] show detail - Cytochrome P450 superfamily protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Cytochrome P450 1.4e-61
    HMMPanther CYTOCHROME P450 90A1 (STEROID 22 ALPHA-HYDROX 2.1e-247
    HMMPanther Cytochrome P450 2.1e-247
    FPrintScan Cytochrome P450, E-class, group I 2.7e-18
    FPrintScan Cytochrome P450, E-class, group I 2.7e-18
    FPrintScan Cytochrome P450, E-class, group I 2.7e-18
    FPrintScan Cytochrome P450, E-class, group I 2.7e-18
    FPrintScan Cytochrome P450, E-class, group I 2.7e-18
    FPrintScan Cytochrome P450, E-class, group I 2.7e-18
    FPrintScan Cytochrome P450, E-class, group I 2.7e-18
    FPrintScan Cytochrome P450, C-terminal 9.1e-10
    FPrintScan Cytochrome P450, C-terminal 9.1e-10
    FPrintScan Cytochrome P450, C-terminal 9.1e-10
    FPrintScan Cytochrome P450, C-terminal 9.1e-10
    PatternScan Cytochrome P450, conserved site 0
    Gene3D Cytochrome P450 1.5e-92
    superfamily Cytochrome P450 2.6e-96

    See the detailed result >

    AT5G05690.1

    Model type
    Protein coding
    Short Description
    Cytochrome P450 superfamily protein
    Curator Summary
    Encodes a member of the CP90A family, a cytochrome P450 monooxygenase which converts 6-deoxocathasterone to 6-deoxoteasterone in the late C6 oxidation pathway and cathasterone to teasterone in the early C6 oxidation pathway of brassinolide biosynthesis. Expressed in cotyledons and leaves. Mutants display de-etiolation and derepression of light-induced genes in the dark, dwarfism, male sterility and activation of stress-regulated genes in the light. The expression of the gene using a CPD promoter:LUC fusion construct was shown to be under circadian and light control. Additionally, the circadian regulation was shown to be independent of BR levels as it remains unchanged in bri1 mutant lines. CPD appears to be involved in the autonomous pathway that regulates the transition to flowering, primarily through a BRI1-mediated signaling pathway that affects FLC expression levels, as uncovered by double mutant analyses.
    Computational Description
    CONSTITUTIVE PHOTOMORPHOGENIC DWARF (CPD); FUNCTIONS IN: electron carrier activity, monooxygenase activity, iron ion binding, oxygen binding, heme binding; INVOLVED IN: in 9 processes; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT3G50660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR