F25546

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F25546

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F25546

Determination of introduced cDNA(s)

  • RAFL05-20-O23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-20-O23 - Resource: pda07458

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-20-O23 - full reading 
    ACCTTTCCCT CACCTGATAT TTATTTTCTT ACCTTAAATA CGACGGTGCT 
    TCACTGAGTC CGACTCAGTT AACTCGTTCC TCTCTCTGTG TGTGGTTTTG 
    GTAGACGACG ACGACGATAA TGGAGGAGCT AGATCGTTCA CGTACTTTTG 
    CCAGAGACGT CAAACGTATC GTCGTTAAGG TTGGGACAGC AGTTGTTACT 
    GGAAAAGGTG GAAGATTGGC TCTTGGTCGT TTAGGAGCAC TGTGTGAACA 
    GCTTGCGGAA TTAAACTCGG ATGGATTTGA GGTGATATTG GTGTCATCTG 
    GTGCGGTTGG TCTTGGCAGG CAAAGGCTTC GTTATCGACA ATTAGTCAAT 
    AGCAGCTTTG CGGATCTTCA GAAGCCTCAG ACTGAACTTG ATGGGAAGGC 
    TTGTGCTGGT GTTGGACAAA GCAGTCTTAT GGCTTACTAT GAGACTATGT 
    TTGACCAGCT TGATGTGACG GCAGCTCAAC TTCTGGTGAA TGACAGTAGT 
    TTTAGAGACA AGGATTTCAG GAAGCAACTT AATGAAACTG TCAAGTCTAT 
    GCTTGATTTG AGGGTTATTC CAATTTTCAA TGAGAATGAT GCTATTAGCA 
    CCCGAAGAGC CCCATATCAG GATTCTTCTG GTATTTTCTG GGATAACGAT 
    AGCTTAGCTG CTCTACTGGC GTTGGAACTG AAAGCTGATC TTCTGATTCT 
    TCTGAGCGAT GTTGAAGGTC TTTACACAGG CCCTCCAAGT GATCCTAACT 
    CAAAGTTGAT CCACACTTTT GTTAAAGAAA AACATCAAGA TGAGATTACA 
    TTCGGCGACA AATCAAGATT AGGGAGAGGG GGTATGACTG CAAAAGTCAA 
    AGCTGCAGTC AATGCAGCTT ATGCTGGGAT TCCTGTCATC ATAACCAGTG 
    GGTATTCAGC TGAGAACATA GATAAAGTCC TCAGAGGACT ACGTGTTGGA 
    ACCTTGTTTC ATCAAGATGC TCGTTTATGG GCTCCGATCA CAGATTCTAA 
    TGCTCGTGAC ATGGCAGTTG CTGCGAGGGA AAGTTCCAGA AAGCTTCAGG 
    CCTTATCTTC GGAAGACAGG AAAAAAATTC TGCTTGATAT TGCCGATGCC 
    CTTGAAGCAA ATGTTACTAC AATCAAAGCT GAGAATGAGT TAGATGTAGC 
    TTCTGCACAA GAGGCTGGGT TGGAAGAGTC AATGGTGGCT CGCTTAGTTA 
    TGACACCTGG AAAGATCTCG AGCCTTGCAG CTTCAGTTCG TAAGCTAGCT 
    GATATGGAAG ATCCAATCGG CCGTGTTTTA AAGAAAACAG AGGTGGCAGA 
    TGGTCTTGTC TTAGAGAAGA CCTCATCACC ATTAGGCGTA CTTCTGATTG 
    TTTTTGAATC CCGACCTGAT GCACTTGTAC AGATAGCTTC ACTTGCCATC 
    CGTAGTGGAA ATGGTCTTCT GCTGAAGGGT GGAAAGGAGG CCCGGCGATC 
    AAATGCTATC TTACACAAGG TGATCACTGA TGCAATTCCA GAGACTGTTG 
    GGGGTAAACT CATTGGACTT GTGACTTCAA GAGAAGAGAT TCCTGATTTG 
    CTTAAGCTTG ATGACGTTAT CGATCTTGTG ATCCCAAGAG GAAGCAACAA 
    GCTTGTTACT CAGATAAAAA ATACTACAAA AATCCCTGTG CTAGGTCATG 
    CTGATGGAAT CTGTCATGTA TATGTCGACA AGGCTTGTGA TACGGATATG 
    GCAAAGCGCA TAGTTTCTGA TGCAAAGTTG GACTATCCAG CAGCCTGTAA 
    TGCGATGGAA ACCCTTCTTG TGCATAAGGA TCTAGAGCAG AATGCTGTGC 
    TTAATGAGCT TATTTTTGCT CTGCAGAGCA ATGGAGTCAC TTTGTATGGT 
    GGACCAAGGG CAAGTAAGAT ACTGAACATA CCAGAAGCAC GGTCATTCAA 
    CCATGAGTAC TGTGCCAAGG CTTGCACTGT TGAAGTTGTA GAAGACGTTT 
    ATGGTGCTAT AGATCACATT CACCGACATG GGAGTGCACA CACAGACTGC 
    ATTGTGACAG AGGATCACGA AGTTGCAGAG CTATTCCTTC GCCAAGTGGA 
    TAGCGCTGCT GTGTTCCACA ACGCCAGCAC AAGATTCTCA GATGGTTTCC 
    GATTTGGACT TGGTGCAGAG GTGGGGGTAA GCACGGGCAG GATCCATGCT 
    CGTGGTCCAG TCGGGGTCGA AGGATTACTT ACAACGAGAT GGATAATGAG 
    AGGAAAAGGA CAAGTTGTCG ACGGAGACAA TGGAATTGTT TACACCCATC 
    AGGACATTCC CATCCAAGCT TAAACAAGAC TTCCGAGTGT GTGTTTGTGT 
    ATTTGGTTGA GACTTGAGGA GAGACACAGA GGAGGATGGG CTTTTTTGTT 
    TCCTCTCTGC TTAGTACTCA TATCCTATCA TTATTATTAT TACTACTACT 
    TATTATTGAA ACCCTCGCTT ATGTAGTGGT TTTGATTTAG GGTTAGGATT 
    GCACCAAAAA TAAGATCCAC TTTACCACTT AGTCTTGCTC ATAAGTACGA 
    TGAAGAACAT TTAATTAGCT TCTCTTCTTG TCATTGTAAG CTACCTACAC 
    ATTTCTGATC TTTATCAAGA TACTACTACT TTTCAAAAAA AAAAAAAAAA

    Gene models with high sequence identity

    1. AT2G39800.1 [+] show detail - delta1-pyrroline-5-carboxylate synthase 1
      E-value: 0; Score: 99.96

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Glutamate 5-kinase, conserved site 0
    PatternScan Gamma-glutamyl phosphate reductase GPR, conserved site 0
    HMMPanther DELTA 1-PYRROLINE-5-CARBOXYLATE SYNTHETASE 0
    HMMPanther GLUTAMATE SEMIALDEHYDE DEHYDROGENASE 0
    HMMPfam Aspartate/glutamate/uridylate kinase 3.9e-37
    HMMPfam Aldehyde dehydrogenase 6.9e-08
    HMMTigr Delta l-pyrroline-5-carboxylate synthetase 0
    HMMTigr Glutamate 5-kinase, ProB-related 7e-26
    HMMTigr Gamma-glutamyl phosphate reductase GPR 8.9e-161
    Gene3D Aspartate/glutamate/uridylate kinase 9.9e-18
    Gene3D Aldehyde dehydrogenase, N-terminal 5.9e-56
    Gene3D Aldehyde dehydrogenase, C-terminal 3.6e-64
    superfamily Aldehyde/histidinol dehydrogenase 4.9e-99
    superfamily Aspartate/glutamate/uridylate kinase 3.7e-53
    HMMPIR Delta l-pyrroline-5-carboxylate synthetase 0
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56

    See the detailed result >

    AT2G39800.1

    Model type
    Protein coding
    Short Description
    delta1-pyrroline-5-carboxylate synthase 1
    Curator Summary
    encodes a delta1-pyrroline-5-carboxylate synthase that catalyzes the rate-limiting enzyme in the biosynthesis of proline. Gene is expressed in reproductive organs and tissues under non-stress conditions but in the whole plant under water-limiting condition. Expression is also induced by abscisic acid and salt stress in a light-dependent manner. P5CS1 appears to be involved in salt stress responses related to proline accumulation, including protection from reactive oxidative species. P5CS1 appears to be present in different cells and/or different subcellular locations from P5CS2 in a tissue-dependent manner.
    Computational Description
    delta1-pyrroline-5-carboxylate synthase 1 (P5CS1); FUNCTIONS IN: delta1-pyrroline-5-carboxylate synthetase activity; INVOLVED IN: in 9 processes; LOCATED IN: chloroplast, membrane, cytoplasm; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: C globular stage, D bilateral stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Glutamate 5-kinase (InterPro:IPR001057), Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Glutamate 5-kinase, conserved site (InterPro:IPR019797), Aldehyde dehydrogenase, N-terminal (InterPro:IPR016162), Gamma-glutamyl phosphate reductase GPR, conserved site (InterPro:IPR020593), Aldehyde dehydrogenase, C-terminal (InterPro:IPR016163), Aldehyde/histidinol dehydrogenase (InterPro:IPR016161), Delta l-pyrroline-5-carboxylate synthetase (InterPro:IPR005766), Gamma-glutamyl phosphate reductase GPR (InterPro:IPR000965), Aldehyde dehydrogenase (InterPro:IPR015590), Glutamate 5-kinase, ProB-related (InterPro:IPR005715); BEST Arabidopsis thaliana protein match is: delta 1-pyrroline-5-carboxylate synthase 2 (TAIR:AT3G55610.1); Has 14932 Blast hits to 14924 proteins in 2380 species: Archae - 163; Bacteria - 9254; Metazoa - 175; Fungi - 266; Plants - 182; Viruses - 0; Other Eukaryotes - 4892 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR