F25829

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F25829

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F25829

Determination of introduced cDNA(s)

  • RAFL04-17-O24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-O24 - Resource: pda00651

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-17-O24 - full reading 
    ATTTGCTCAC CTTTCGCCGA TTGATTTTGT ACATCACACG CAGACGCAGC 
    TTTTAGTATT TTTTTCAACA ATCGCTTTTT TTTTTTTCTC GATCTCCGTG 
    AAGCTCTCGA ATCTCTGGTT TTGAGTTTTG GAGAATGGTG TTTTTCCGCA 
    GCGTATCGGC CTTTACTAGG CTGAGGTCTC GCGTCGGCCA ACAATCTTCA 
    CTCAGCAATT CTGTCAGATG GATTCAGATG CAGAGCTCTA CCGACCTGGA 
    CCTGAAGTCG CAGCTGCAAG AGTTAATTCC GGAACAGCAG GACCGTCTGA 
    AGAAACTGAA GTCAGAACAT GGGAAGGTCC AACTGGGAAA CATCACTGTT 
    GATATGGTTA TTGGTGGAAT GAGAGGGATG ACTGGATTGC TTTGGGAAAC 
    CTCATTGCTT GACCCGGAAG AGGGTATTCG CTTTAGGGGA TTGTCGATTC 
    CTGAGTGCCA GAAAGTATTA CCTACTGCCC AGTCTGGAGC TGAACCATTA 
    CCGGAGGGTC TTTTGTGGCT TCTCTTAACT GGAAAGGTAC CTAGCAAAGA 
    GCAAGTTGAA GCACTGTCGA AAGACTTGGC GAACCGTGCT GCTGTGCCAG 
    ATTATGTGTA CAATGCCATC GATGCTCTGC CTTCCACAGC TCATCCAATG 
    ACTCAATTTG CTAGCGGTGT TATGGCCCTC CAGGTGCAAA GTGAGTTTCA 
    AAAGGCATAT GAGAATGGAA TTCATAAGTC AAAGTTCTGG GAGCCAACAT 
    ATGAGGATTG CCTCAACCTG ATTGCTCGTG TTCCTGTTGT AGCTGCATAT 
    GTTTATCGAA GGATGTATAA GAATGGTGAT TCCATTCCCT CAGATAAATC 
    TTTGGATTAT GGTGCAAATT TTTCCCACAT GTTGGGATTT GATGATGAAA 
    AGGTGAAAGA GCTCATGAGG CTTTACATCA CCATCCACAG TGATCATGAA 
    GGTGGAAATG TTAGTGCTCA CACTGGTCAC CTGGTTGGTA GTGCACTTTC 
    AGACCCATAT CTGTCATTTG CAGCTGCATT AAATGGTTTA GCTGGGCCAC 
    TCCATGGTTT GGCTAATCAG GAAGTTTTGC TTTGGATCAA ATCAGTCGTA 
    GAGGAATGTG GAGAAGATAT ATCAAAAGAA CAGTTGAAAG AATATGTTTG 
    GAAAACATTA AACAGTGGCA AGGTTATTCC GGGATATGGT CACGGTGTTC 
    TGCGCAATAC TGATCCAAGA TATGTATGCC AAAGAGAATT TGCCTTGAAG 
    CATCTACCTG ACGACCCTCT TTTCCAGCTG GTGTCAAAGC TTTATGAAGT 
    TGTGCCTCCT GTTCTCACTG AGCTTGGAAA GGTGAAGAAC CCGTGGCCAA 
    ATGTTGATGC TCACAGTGGG GTCTTGCTGA ACCACTATGG TCTAACCGAA 
    GCAAGGTACT ACACCGTGCT CTTTGGTGTT TCAAGGAGTC TTGGCATCTG 
    CTCTCAGCTT ATATGGGACC GAGCTCTTGG ACTTGCACTT GAGAGGCCAA 
    AGAGTGTTAC CATGGACTGG CTTGAAGCCC ATTGTAAGAA AGCTTCATCT 
    GCTTAAGTCA CCACCGAGTC CTGAGTTGTC GCACTCTCTC GTGTTTCAAC 
    AAAAGTCATT CTCTTCCACA CTGGATAGCT TTTTTTGTTT TTACCTTCTA 
    TTGACAGAAG AAACATTTGT TCATGTAGAA ATTGTTCCTG AGAAACACAT 
    GCTTGGTGCA AATTTGCATT TCCGGGAAAC TCGTGATAAT AAATAAACAG 
    TTGTATT

    Gene models with high sequence identity

    1. AT2G44350.1 [+] show detail - Citrate synthase family protein
      E-value: 0; Score: 99.89

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Citrate synthase, eukaryotic 0
    superfamily Citrate synthase-like, core 2e-145
    Gene3D Citrate synthase-like, large alpha subdomain 9.1e-101
    PatternScan Citrate synthase active site 0
    HMMPanther Citrate synthase-like 5.4e-217
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    HMMPfam Citrate synthase-like 1.2e-106
    Coil coiled-coil 0

    See the detailed result >

    AT2G44350.1

    Model type
    Protein coding
    Short Description
    Citrate synthase family protein
    Curator Summary
    encodes a mitochrondrion targeted citrate synthase, the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate, finally yielding citrate and CoA.
    Computational Description
    ATCS; FUNCTIONS IN: citrate (SI)-synthase activity, zinc ion binding, ATP binding; INVOLVED IN: response to cadmium ion, tricarboxylic acid cycle; LOCATED IN: mitochondrion, cell wall, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Citrate synthase-like, large alpha subdomain (InterPro:IPR016142), Citrate synthase, eukaryotic (InterPro:IPR010109), Citrate synthase active site (InterPro:IPR019810), Citrate synthase-like (InterPro:IPR002020), Citrate synthase-like, core (InterPro:IPR016141); BEST Arabidopsis thaliana protein match is: citrate synthase 5 (TAIR:AT3G60100.1); Has 10534 Blast hits to 10528 proteins in 2768 species: Archae - 158; Bacteria - 6911; Metazoa - 258; Fungi - 320; Plants - 178; Viruses - 0; Other Eukaryotes - 2709 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR