F26227

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F26227

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F26227

Determination of introduced cDNA(s)

  • RAFL05-18-L20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-18-L20 - Resource: pda17126

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-18-L20 - full reading
    CCGAAGTGAA TTCGATTTAC ACTTTTTTGT CCTTTTTTAT TAATAAATCA 
    CTGACCCGAA AATAAAAATA GAAGCAAAAC TTCATGTCGT GGTTTCTAAT
    AGCGGTGGCG ACAATCGCCG CCGTCGTATC CTACAAGCTA ATCCAACGGC
    TAAGATACAA GTTCCCACCA GGCCCAAGCC CCAAGCCGAT CGTCGGTAAC
    CTCTACGACA TAAAACCGGT CCGGTTCAGA TGTTACTACG AGTGGGCTCA
    ATCTTATGGA CCAATCATAT CGGTCTGGAT CGGTTCAATT CTAAACGTGG
    TTGTATCTAG CGCCGAGCTA GCAAAAGAAG TTCTGAAAGA ACACGACCAG
    AAACTCGCCG ACCGGCACCG GAACAGATCG ACGGAAGCAT TTAGCCGCAA
    CGGTCAGGAT CTTATATGGG CCGATTATGG GCCTCATTAC GTGAAGGTGA
    GAAAAGTTTG CACGCTTGAG CTCTTCACAC CGAAACGACT CGAGTCTCTC
    AGACCTATCC GTGAAGATGA AGTCACCGCC ATGGTTGAAT CCGTCTTCAG
    AGACTGTAAC CTTCCTGAAA ACAGAGCAAA AGGTTTACAA CTGAGGAAGT
    ACTTAGGAGC GGTTGCGTTC AACAACATAA CGCGGCTAGC CTTTGGGAAG
    CGTTTTATGA ACGCTGAAGG TGTTGTGGAC GAGCAAGGGC TTGAGTTCAA
    GGCCATAGTA TCCAACGGTC TGAAGCTAGG TGCTTCACTG TCAATAGCTG
    AACACATCCC GTGGCTCAGG TGGATGTTTC CGGCTGATGA GAAGGCGTTT
    GCTGAGCACG GGGCTCGTCG TGACCGCCTC ACTCGAGCTA TCATGGAGGA
    GCATACTTTG GCCCGTCAAA AGTCTAGTGG AGCGAAACAG CATTTCGTTG
    ATGCGTTGCT AACGTTGAAG GATCAGTATG ATCTTAGTGA GGATACTATC
    ATTGGTCTTC TATGGGATAT GATCACGGCA GGGATGGACA CGACAGCGAT
    AACAGCGGAA TGGGCGATGG CGGAAATGAT CAAGAATCCA AGAGTGCAAC
    AAAAAGTGCA AGAAGAGTTC GACAGAGTGG TTGGACTTGA CCGGATCTTA
    ACCGAGGCAG ATTTCTCCCG CTTACCTTAC TTGCAATGCG TGGTGAAAGA
    GTCATTCAGG CTGCATCCTC CAACGCCTCT AATGCTACCT CACCGAAGCA
    ACGCAGATGT CAAGATCGGA GGCTATGATA TTCCCAAAGG ATCAAACGTT
    CATGTGAATG TGTGGGCTGT GGCTAGAGAC CCGGCTGTAT GGAAAAATCC
    ATTTGAGTTT AGACCAGAGA GATTCTTGGA AGAAGATGTT GACATGAAGG
    GTCATGATTT TAGGCTGCTT CCGTTTGGAG CTGGAAGACG GGTTTGTCCC
    GGTGCACAAC TTGGTATCAA TTTGGTAACT TCGATGATGA GTCATTTGCT
    TCACCATTTT GTTTGGACAC CTCCTCAAGT GACTAAACCG GAGGAGATTG
    ACATGTCTGA AAACCCTGGA CTCGTTACTT ACATGCGTAC CCCTGTGCAA
    GCGGTTGCAA CGCCTCGGTT GCCTTCGGAT CTGTACAAAC GCGTGCCTTA
    CGATATGTAA ATGTCACTCT GATCTACCTT TTGTTGCTGC TGCTCATGCT
    CTTGTTGTTG TTTGTAGACA TGTTTCTTGT GGTTTATCAT CGAAAAACTT
    TTGATGAGAT TTGTTGTATC AATTCTCTCA AGGAGAAGTA AAGAAGCCAT
    CTTCTTTGTC GAAAAAAAAA A

    Gene models with high sequence identity

    1. AT2G40890.1 [+] show detail - cytochrome P450, family 98, subfamily A, polypeptide 3
      E-value: 0; Score: 99.77

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, C-terminal 1.4e-09
    FPrintScan Cytochrome P450, C-terminal 1.4e-09
    FPrintScan Cytochrome P450, C-terminal 1.4e-09
    HMMPfam Cytochrome P450 2.4e-102
    superfamily Cytochrome P450 3e-128
    HMMPanther CYTOCHROME P450, SUBFAMILY 98A 0
    HMMPanther Cytochrome P450 0
    PatternScan Cytochrome P450, conserved site 0
    Gene3D Cytochrome P450 1e-120

    See the detailed result >

    AT2G40890.1

    Model type
    Protein coding
    Short Description
    cytochrome P450, family 98, subfamily A, polypeptide 3
    Curator Summary
    encodes coumarate 3-hydroxylase (C3H), a P450-dependent monooxygenase. Involved in lignin biosynthesis and flavonoid biosynthesis. Also affects the biosynthesis of coumarins such as scopoletin and scopolin as a branching-out-pathway from the phenylpropanoid acid level.
    Computational Description
    cytochrome P450, family 98, subfamily A, polypeptide 3 (CYP98A3); FUNCTIONS IN: monooxygenase activity, p-coumarate 3-hydroxylase activity; INVOLVED IN: coumarin biosynthetic process, lignin biosynthetic process, phenylpropanoid biosynthetic process, flavonoid biosynthetic process; LOCATED IN: mitochondrion, endoplasmic reticulum, plasma membrane, microsome; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 98, subfamily A, polypeptide 9 (TAIR:AT1G74550.1); Has 33899 Blast hits to 33682 proteins in 1752 species: Archae - 48; Bacteria - 3997; Metazoa - 11870; Fungi - 7164; Plants - 9566; Viruses - 3; Other Eukaryotes - 1251 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR