F26227

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F26227

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F26227

Determination of introduced cDNA(s)

  • RAFL05-18-L20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-18-L20 - Resource: pda17126

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-18-L20 - full reading 
    CCGAAGTGAA TTCGATTTAC ACTTTTTTGT CCTTTTTTAT TAATAAATCA 
    CTGACCCGAA AATAAAAATA GAAGCAAAAC TTCATGTCGT GGTTTCTAAT 
    AGCGGTGGCG ACAATCGCCG CCGTCGTATC CTACAAGCTA ATCCAACGGC 
    TAAGATACAA GTTCCCACCA GGCCCAAGCC CCAAGCCGAT CGTCGGTAAC 
    CTCTACGACA TAAAACCGGT CCGGTTCAGA TGTTACTACG AGTGGGCTCA 
    ATCTTATGGA CCAATCATAT CGGTCTGGAT CGGTTCAATT CTAAACGTGG 
    TTGTATCTAG CGCCGAGCTA GCAAAAGAAG TTCTGAAAGA ACACGACCAG 
    AAACTCGCCG ACCGGCACCG GAACAGATCG ACGGAAGCAT TTAGCCGCAA 
    CGGTCAGGAT CTTATATGGG CCGATTATGG GCCTCATTAC GTGAAGGTGA 
    GAAAAGTTTG CACGCTTGAG CTCTTCACAC CGAAACGACT CGAGTCTCTC 
    AGACCTATCC GTGAAGATGA AGTCACCGCC ATGGTTGAAT CCGTCTTCAG 
    AGACTGTAAC CTTCCTGAAA ACAGAGCAAA AGGTTTACAA CTGAGGAAGT 
    ACTTAGGAGC GGTTGCGTTC AACAACATAA CGCGGCTAGC CTTTGGGAAG 
    CGTTTTATGA ACGCTGAAGG TGTTGTGGAC GAGCAAGGGC TTGAGTTCAA 
    GGCCATAGTA TCCAACGGTC TGAAGCTAGG TGCTTCACTG TCAATAGCTG 
    AACACATCCC GTGGCTCAGG TGGATGTTTC CGGCTGATGA GAAGGCGTTT 
    GCTGAGCACG GGGCTCGTCG TGACCGCCTC ACTCGAGCTA TCATGGAGGA 
    GCATACTTTG GCCCGTCAAA AGTCTAGTGG AGCGAAACAG CATTTCGTTG 
    ATGCGTTGCT AACGTTGAAG GATCAGTATG ATCTTAGTGA GGATACTATC 
    ATTGGTCTTC TATGGGATAT GATCACGGCA GGGATGGACA CGACAGCGAT 
    AACAGCGGAA TGGGCGATGG CGGAAATGAT CAAGAATCCA AGAGTGCAAC 
    AAAAAGTGCA AGAAGAGTTC GACAGAGTGG TTGGACTTGA CCGGATCTTA 
    ACCGAGGCAG ATTTCTCCCG CTTACCTTAC TTGCAATGCG TGGTGAAAGA 
    GTCATTCAGG CTGCATCCTC CAACGCCTCT AATGCTACCT CACCGAAGCA 
    ACGCAGATGT CAAGATCGGA GGCTATGATA TTCCCAAAGG ATCAAACGTT 
    CATGTGAATG TGTGGGCTGT GGCTAGAGAC CCGGCTGTAT GGAAAAATCC 
    ATTTGAGTTT AGACCAGAGA GATTCTTGGA AGAAGATGTT GACATGAAGG 
    GTCATGATTT TAGGCTGCTT CCGTTTGGAG CTGGAAGACG GGTTTGTCCC 
    GGTGCACAAC TTGGTATCAA TTTGGTAACT TCGATGATGA GTCATTTGCT 
    TCACCATTTT GTTTGGACAC CTCCTCAAGT GACTAAACCG GAGGAGATTG 
    ACATGTCTGA AAACCCTGGA CTCGTTACTT ACATGCGTAC CCCTGTGCAA 
    GCGGTTGCAA CGCCTCGGTT GCCTTCGGAT CTGTACAAAC GCGTGCCTTA 
    CGATATGTAA ATGTCACTCT GATCTACCTT TTGTTGCTGC TGCTCATGCT 
    CTTGTTGTTG TTTGTAGACA TGTTTCTTGT GGTTTATCAT CGAAAAACTT 
    TTGATGAGAT TTGTTGTATC AATTCTCTCA AGGAGAAGTA AAGAAGCCAT 
    CTTCTTTGTC GAAAAAAAAA A

    Gene models with high sequence identity

    1. AT2G40890.1 [+] show detail - cytochrome P450, family 98, subfamily A, polypeptide 3
      E-value: 0; Score: 99.77

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, E-class, group I 1.1e-49
    FPrintScan Cytochrome P450, C-terminal 0.0000000014
    FPrintScan Cytochrome P450, C-terminal 0.0000000014
    FPrintScan Cytochrome P450, C-terminal 0.0000000014
    HMMPfam Cytochrome P450 2.4e-102
    superfamily Cytochrome P450 3e-128
    HMMPanther CYTOCHROME P450, SUBFAMILY 98A 0
    HMMPanther Cytochrome P450 0
    PatternScan Cytochrome P450, conserved site 0
    Gene3D Cytochrome P450 1e-120

    See the detailed result >

    AT2G40890.1

    Model type
    Protein coding
    Short Description
    cytochrome P450, family 98, subfamily A, polypeptide 3
    Curator Summary
    encodes coumarate 3-hydroxylase (C3H), a P450-dependent monooxygenase. Involved in lignin biosynthesis and flavonoid biosynthesis. Also affects the biosynthesis of coumarins such as scopoletin and scopolin as a branching-out-pathway from the phenylpropanoid acid level.
    Computational Description
    cytochrome P450, family 98, subfamily A, polypeptide 3 (CYP98A3); FUNCTIONS IN: monooxygenase activity, p-coumarate 3-hydroxylase activity; INVOLVED IN: coumarin biosynthetic process, lignin biosynthetic process, phenylpropanoid biosynthetic process, flavonoid biosynthetic process; LOCATED IN: mitochondrion, endoplasmic reticulum, plasma membrane, microsome; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 98, subfamily A, polypeptide 9 (TAIR:AT1G74550.1); Has 33899 Blast hits to 33682 proteins in 1752 species: Archae - 48; Bacteria - 3997; Metazoa - 11870; Fungi - 7164; Plants - 9566; Viruses - 3; Other Eukaryotes - 1251 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR