F26411

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F26411

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Original: F26411

Determination of introduced cDNA(s)

  • RAFL05-18-H15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-18-H15 - Resource: pda17124

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-18-H15 - full reading
    CCGGTTAACA CCTAGGTTCT TGTTTCCTAA CAAAGGCTCT ACTTTTTCTT 
    TAGTTAACTA CTATGGCTCA TTTCCTCGAG ACACAGGAGC CCCTCGTATT
    CTCCGGGAAG AAACGAAACG ATCGCGATGA CGAAGACGGT GACGCCTTGG
    TTGCGAAGAA ATCAGCACTG GCGGTGTGTG ATGCCGACCC TGCAGCGGCC
    ATAGCGAATA TCCGACATGA GTTTGGGGAG CACGGGGGAG TAAACATGTC
    CATCGAAGCC TCCGCCACCT TCACCGTCAT GGAACCGGAT ACCATGAGGC
    GGATGTTCAC CGGAGAACTG GGACCGGACA ACGACTTCTA CGTATACAGC
    CGACACTTCA ACCCCACCGT GCTCAACCTC AGCCGCCAGA TGGCTGCTCT
    CGAAGGCACC CAAGCTGCCT ACTGTACCTC TAGCGGTATG TCGGCGATAT
    CGTCAGTGAT GCTGCAACTG TGCAGCAGCG GAGGACACGT GGTTGCGGCA
    AGCACGCTAT ACGGAGGAAC ACACGCTTTG CTCTCTCATT TCTTGCCACG
    GACATGTAAC ATAACCACCT CCTTCGTTGA CATAACGGAC CATGGCGCCG
    TGGCAAACGC GATCGTTGAG GGTAGGACAC AGGTTCTCTA CTTTGAGTCG
    GTGGCAAACC CGACGCTGAC TGTGGCTGAC ATACCTGAGC TGAGCCGTAT
    GGCACACGAG AAGGGCGTGA CGGTGGTGGT GGACAACACA TTCGCCCCCA
    TGGTGCTGTC TCCGGCCAAG CTTGGAGCAG ATGTGGTGGT TCACAGTATC
    TCCAAGTTCA TCAGTGGTGG GGCTGACATC ATCGCAGGGG CCGTGTGTGG
    GAGTGAGAAT TTGGTGAAAG AGATGATGGA TCTTCGTGGC GGATCTCTGA
    TGCTTCTAGG TCCCACCATG AACGCCAAGG TGGCTTTCGA GCTCTCCGAG
    CGAATCCCTC ACTTGGGCCT ACGCATGAGA GAGCACAGCC ACAGAGCCCA
    AGTGTATGCG GAGAGAATGA GGGATTTGGG CATGAAAGTC ATATATCCAG
    GGCTCGAGAC CCACCCGCAG CACAAGCTAT TCAAAGGGAT GGTGAATAGA
    GACTATGGAT ACGGAGGATT GCTGTCGATA GACATGGAGA CAGAGGAGAA
    AGCCAACAAG CTCATGGCAT ATCTCCAGAA CGCCACTCAG TTTGGCTTCA
    TGGCCGTCAG TTTGGGTTAC TACGAGACAC TCATGTCTTG CTCCGGGAGC
    AGCACTAGCA GCGAGCTTGA CCCCTCACAG AAGGAAGCTG CAGGCATCTC
    TCCTGGTCTT GTACGAATGT CGGTGGGGTA TGTGGGTACA TTGGAGCAGA
    AGTGGACACA ATTCGAGAAA GCATTCCTCA GAATGTAAAA TGTCTTCTTC
    TATATATGAT GGTTTTCTTT CCCCCAAATA CCAACAATTC ATCATCTGCG
    TTATATGTAT GATGTGGCCA ATGTTCAGTC TCTTATTTGT TATATCACAA
    TGTTCTAGTC TTCGGTAAAA AAAAAA

    Gene models with high sequence identity

    1. AT1G64660.1 [+] show detail - methionine gamma-lyase
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPIR Cys/Met metabolism, pyridoxal phosphate-dependent enzyme 5.7e-118
    HMMPanther METHIONINE GAMMA-LYASE 5.6e-221
    HMMPanther Cys/Met metabolism, pyridoxal phosphate-dependent enzyme 5.6e-221
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 3e-57
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 2 8.6e-19
    HMMPfam Cys/Met metabolism, pyridoxal phosphate-dependent enzyme 8.2e-88
    superfamily Pyridoxal phosphate-dependent transferase, major domain 1.2e-80

    See the detailed result >

    AT1G64660.1

    Model type
    Protein coding
    Short Description
    methionine gamma-lyase
    Curator Summary
    Encodes a functional methionine gamma-lyase, a cytosolic enzyme catalyzes the degradation of methionine into methanethiol, alpha-ketobutyrate and ammonia. The catabolism of excess methionine is important to methionine homeostasis.
    Computational Description
    methionine gamma-lyase (MGL); CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cys/Met metabolism, pyridoxal phosphate-dependent enzyme (InterPro:IPR000277), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (InterPro:IPR015422); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT3G01120.1); Has 19547 Blast hits to 19544 proteins in 2474 species: Archae - 216; Bacteria - 11847; Metazoa - 211; Fungi - 771; Plants - 270; Viruses - 0; Other Eukaryotes - 6232 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR