F26921

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F26921

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F26921

Determination of introduced cDNA(s)

  • RAFL05-02-I23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-02-I23 - Resource: pda00720

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-02-I23 - full reading 
    ATAAAAAGTA GTACTCGTGT CTTACTCGTG CCAGCCACTC GCATTTCTCC 
    AGATTTTATT ATCCTTCCTC GAAACAAGAG CTTAGCTAAG ATGACGAAGA 
    ACTACCCAAC CGTGAGCGAA GATTACAAGA AGGCTGTTGA GAAGTGCAGG 
    AGGAAGCTCA GAGGTTTGAT CGCTGAGAAG AACTGTGCAC CCATCATGGT 
    CCGACTCGCA TGGCACTCTG CTGGAACTTT CGATTGTCAA TCAAGGACTG 
    GAGGTCCATT CGGAACAATG AGGTTTGACG CTGAGCAAGC TCATGGAGCC 
    AACAGTGGTA TCCACATTGC TCTTAGGTTG TTGGACCCCA TCAGGGAGCA 
    ATTCCCTACC ATCTCTTTTG CTGATTTCCA TCAGCTTGCT GGTGTTGTGG 
    CCGTTGAAGT TACTGGTGGC CCTGACATTC CTTTCCACCC TGGAAGAGAG 
    GACAAGCCCC AACCACCTCC AGAGGGTCGT CTTCCTGATG CTACCAAGGG 
    TTGTGACCAT TTGAGAGATG TCTTTGCTAA GCAGATGGGC TTATCTGACA 
    AAGACATTGT CGCTTTATCT GGTGCCCACA CTCTGGGACG ATGCCACAAG 
    GATAGGTCTG GCTTCGAAGG TGCATGGACA TCAAACCCTC TAATCTTCGA 
    CAACTCTTAC TTCAAGGAAC TCTTGAGCGG AGAGAAGGAA GGCCTTCTTC 
    AGCTTGTCTC TGACAAAGCA CTATTGGACG ACCCTGTTTT CCGTCCTTTG 
    GTCGAGAAAT ACGCTGCTGA TGAAGATGCC TTTTTCGCTG ATTACGCTGA 
    GGCCCACATG AAGCTTTCTG AGCTTGGGTT TGCTGATGCT TAAGCTGTGA 
    CGTATGCTCT GTGTGTGTGT GTGTGTCTCC CCGAGAGTCA TGGCTGTTTT 
    TGGTTGGGGG CTGGAGGGGT CGCATTGCAT TTGAACTTTG TCATATGATT 
    GCTTAATGTA CTCTCGGATT TACGTATCTG TTTTTTTCGG TGGGTTGCGA 
    TTTGAACACA TCCGTTGCGC TTTTGCTGCT TAGACACATT AATAAAATTA 
    ATTTCTCCC

    Gene models with high sequence identity

    1. AT1G07890.1 [+] show detail - ascorbate peroxidase 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Haem peroxidase 2.3e-87
    HMMPfam Haem peroxidase, plant/fungal/bacterial 9.7e-47
    ProfileScan Haem peroxidase, plant/fungal/bacterial 16.222
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Plant ascorbate peroxidase 1e-121
    FPrintScan Haem peroxidase, plant/fungal/bacterial 1.9e-26
    FPrintScan Haem peroxidase, plant/fungal/bacterial 1.9e-26
    FPrintScan Haem peroxidase, plant/fungal/bacterial 1.9e-26
    FPrintScan Haem peroxidase, plant/fungal/bacterial 1.9e-26
    FPrintScan Haem peroxidase, plant/fungal/bacterial 1.9e-26
    PatternScan Peroxidases heam-ligand binding site 0
    PatternScan Peroxidase, active site 0
    Gene3D no description 7.4e-45
    Coil coiled-coil 0

    See the detailed result >

    AT1G07890.1

    Model type
    Protein coding
    Short Description
    ascorbate peroxidase 1
    Curator Summary
    Encodes a cytosolic ascorbate peroxidase APX1. Ascorbate peroxidases are enzymes that scavenge hydrogen peroxide in plant cells. Eight types of APX have been described for Arabidopsis: three cytosolic (APX1, APX2, APX6), two chloroplastic types (stromal sAPX, thylakoid tAPX), and three microsomal (APX3, APX4, APX5) isoforms. At least part of the induction of heat shock proteins during light stress in Arabidopsis is mediated by H2O2 that is scavenged by APX1. Expression of the gene is downregulated in the presence of paraquat, an inducer of photoxidative stress.
    Computational Description
    ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, cell wall, chloroplast, plasma membrane, chloroplast stroma; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Peroxidase, active site (InterPro:IPR019794), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 8811 Blast hits to 8057 proteins in 1265 species: Archae - 75; Bacteria - 2912; Metazoa - 21; Fungi - 794; Plants - 3291; Viruses - 0; Other Eukaryotes - 1718 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR