F27141

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27141

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
T2 rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27141

Determination of introduced cDNA(s)

  • RAFL04-17-L07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-L07 - Resource: pda00648

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-17-L07 - full reading 
    ACTACGATAG TTTTCTTCAG CAATCACACT CTCTCTTTGT TCGAGTACCA 
    ACAATGGCCC TTCAAGCTGC TTCTTTGGTC TCCTCTGCTT TCTCTGTTCG 
    CAAAGATGCG AAGTTGAATG CTTCTTCATC ATCTTTCAAG GACTCGAGTC 
    TTTTTGGTGC CTCCATTACC GACCAAATCA AATCCGAACA TGGATCTTCC 
    TCGTTAAGAT TCAAGAGAGA ACAGAGCTTA AGGAATCTAG CAATTCGAGC 
    CCAAACCGCT GCGACTTCAA GCCCTACAGT TACAAAATCC GTGGACGGCA 
    AGAAAACGTT GAGGAAAGGA AATGTGGTGG TCACTGGAGC CTCGTCTGGG 
    TTAGGTCTAG CCACGGCTAA AGCTCTAGCT GAGACAGGGA AATGGAACGT 
    GATAATGGCG TGCAGAGACT TCCTTAAAGC CGAGAGAGCT GCTAAATCCG 
    TAGGGATGCC TAAAGACAGC TACACAGTGA TGCATTTAGA CTTAGCCTCG 
    TTGGACAGCG TGAGACAGTT TGTTGATAAT TTCAGGAGAA CAGAGACGCC 
    TCTCGATGTT TTGGTCTGCA ATGCTGCGGT TTATTTCCCG ACAGCTAAAG 
    AGCCTACTTA CAGTGCTGAA GGGTTTGAGC TTAGTGTTGC GACGAACCAT 
    TTGGGACATT TTCTTCTCGC AAGGTTGTTG CTTGATGACT TGAAGAAATC 
    TGATTACCCT TCAAAGCGTC TCATCATCGT CGGATCCATT ACCGGGAACA 
    CGAATACATT GGCGGGTAAT GTACCACCGA AGGCGAATCT CGGGGATTTG 
    AGGGGTTTAG CCGGCGGATT AAACGGTTTA AACAGCTCAG CTATGATTGA 
    TGGAGGAGAT TTCGACGGTG CAAAGGCTTA CAAAGACAGT AAAGTCTGCA 
    ATATGTTGAC AATGCAAGAG TTTCACAGGC GTTTCCATGA AGAAACTGGA 
    GTCACTTTCG CTTCGCTTTA CCCCGGTTGC ATCGCCTCCA CAGGTTTATT 
    CCGAGAGCAC ATTCCTCTCT TCCGTGCCCT CTTCCCTCCC TTTCAGAAGT 
    ACATCACTAA AGGATATGTC TCCGAAACAG AGTCAGGCAA AAGACTTGCT 
    CAGGTGGTGA GTGATCCAAG CTTGACGAAA TCAGGGGTTT ATTGGAGCTG 
    GAACAATGCT TCGGCTTCTT TTGAGAACCA GTTATCAGAA GAAGCAAGTG 
    ACGTTGAGAA GGCTCGTAAA GTGTGGGAGA TCAGTGAGAA GCTCGTGGGC 
    TTGGCCTAAT AAACTTGAAG TCTGTTAAAT TTGATGACAC CTCTCGGTAC 
    CAATCGAAGA AATCTAGTGA ATTTCCGGGA TTTTTAGATA TCTGATTCTG 
    TTGCGAAGTT TTTTTATCCT TATTAGTGTC TGTTGAGTGT AGATTGCTTG 
    GCGAGTAGCC CACAATAAAG AAATAAAAAT GGCGAATCTT TAGCTTTATT 
    TACTCTGTTT TTCATCGAAA CAGATTCAGT AATATATACT CTGTTTTT

    Gene models with high sequence identity

    1. AT4G27440.1 [+] show detail - protochlorophyllide oxidoreductase B
      E-value: 0; Score: 99.93

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Glucose/ribitol dehydrogenase 3.5e-07
    FPrintScan Glucose/ribitol dehydrogenase 3.5e-07
    FPrintScan Glucose/ribitol dehydrogenase 3.5e-07
    HMMTigr Light-dependent protochlorophyllide reductase 1.4e-251
    HMMPfam Short-chain dehydrogenase/reductase SDR 1.7e-18
    HMMPanther PROTOCHLOROPHYLLIDE REDUCTASE 1.4e-181
    HMMPanther Short-chain dehydrogenase/reductase SDR 1.4e-181
    Gene3D NAD(P)-binding domain 7.2e-41
    superfamily NAD(P)-binding domain 1.3e-40

    See the detailed result >

    AT4G27440.1

    Model type
    Protein coding
    Short Description
    protochlorophyllide oxidoreductase B
    Curator Summary
    light-dependent NADPH:protochlorophyllide oxidoreductase B
    Computational Description
    protochlorophyllide oxidoreductase B (PORB); FUNCTIONS IN: oxidoreductase activity, protochlorophyllide reductase activity; INVOLVED IN: chlorophyll biosynthetic process, response to ethylene stimulus; LOCATED IN: chloroplast thylakoid membrane, chloroplast, membrane, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Light-dependent protochlorophyllide reductase (InterPro:IPR005979), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: protochlorophyllide oxidoreductase A (TAIR:AT5G54190.1); Has 19396 Blast hits to 19385 proteins in 2086 species: Archae - 175; Bacteria - 10514; Metazoa - 1820; Fungi - 1776; Plants - 1038; Viruses - 0; Other Eukaryotes - 4073 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR