F27141

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27141

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
T2 rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27141

Determination of introduced cDNA(s)

  • RAFL04-17-L07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-L07 - Resource: pda00648

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-17-L07 - full reading
    ACTACGATAG TTTTCTTCAG CAATCACACT CTCTCTTTGT TCGAGTACCA 
    ACAATGGCCC TTCAAGCTGC TTCTTTGGTC TCCTCTGCTT TCTCTGTTCG
    CAAAGATGCG AAGTTGAATG CTTCTTCATC ATCTTTCAAG GACTCGAGTC
    TTTTTGGTGC CTCCATTACC GACCAAATCA AATCCGAACA TGGATCTTCC
    TCGTTAAGAT TCAAGAGAGA ACAGAGCTTA AGGAATCTAG CAATTCGAGC
    CCAAACCGCT GCGACTTCAA GCCCTACAGT TACAAAATCC GTGGACGGCA
    AGAAAACGTT GAGGAAAGGA AATGTGGTGG TCACTGGAGC CTCGTCTGGG
    TTAGGTCTAG CCACGGCTAA AGCTCTAGCT GAGACAGGGA AATGGAACGT
    GATAATGGCG TGCAGAGACT TCCTTAAAGC CGAGAGAGCT GCTAAATCCG
    TAGGGATGCC TAAAGACAGC TACACAGTGA TGCATTTAGA CTTAGCCTCG
    TTGGACAGCG TGAGACAGTT TGTTGATAAT TTCAGGAGAA CAGAGACGCC
    TCTCGATGTT TTGGTCTGCA ATGCTGCGGT TTATTTCCCG ACAGCTAAAG
    AGCCTACTTA CAGTGCTGAA GGGTTTGAGC TTAGTGTTGC GACGAACCAT
    TTGGGACATT TTCTTCTCGC AAGGTTGTTG CTTGATGACT TGAAGAAATC
    TGATTACCCT TCAAAGCGTC TCATCATCGT CGGATCCATT ACCGGGAACA
    CGAATACATT GGCGGGTAAT GTACCACCGA AGGCGAATCT CGGGGATTTG
    AGGGGTTTAG CCGGCGGATT AAACGGTTTA AACAGCTCAG CTATGATTGA
    TGGAGGAGAT TTCGACGGTG CAAAGGCTTA CAAAGACAGT AAAGTCTGCA
    ATATGTTGAC AATGCAAGAG TTTCACAGGC GTTTCCATGA AGAAACTGGA
    GTCACTTTCG CTTCGCTTTA CCCCGGTTGC ATCGCCTCCA CAGGTTTATT
    CCGAGAGCAC ATTCCTCTCT TCCGTGCCCT CTTCCCTCCC TTTCAGAAGT
    ACATCACTAA AGGATATGTC TCCGAAACAG AGTCAGGCAA AAGACTTGCT
    CAGGTGGTGA GTGATCCAAG CTTGACGAAA TCAGGGGTTT ATTGGAGCTG
    GAACAATGCT TCGGCTTCTT TTGAGAACCA GTTATCAGAA GAAGCAAGTG
    ACGTTGAGAA GGCTCGTAAA GTGTGGGAGA TCAGTGAGAA GCTCGTGGGC
    TTGGCCTAAT AAACTTGAAG TCTGTTAAAT TTGATGACAC CTCTCGGTAC
    CAATCGAAGA AATCTAGTGA ATTTCCGGGA TTTTTAGATA TCTGATTCTG
    TTGCGAAGTT TTTTTATCCT TATTAGTGTC TGTTGAGTGT AGATTGCTTG
    GCGAGTAGCC CACAATAAAG AAATAAAAAT GGCGAATCTT TAGCTTTATT
    TACTCTGTTT TTCATCGAAA CAGATTCAGT AATATATACT CTGTTTTT

    Gene models with high sequence identity

    1. AT4G27440.1 [+] show detail - protochlorophyllide oxidoreductase B
      E-value: 0; Score: 99.93

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Glucose/ribitol dehydrogenase 3.5e-07
    FPrintScan Glucose/ribitol dehydrogenase 3.5e-07
    FPrintScan Glucose/ribitol dehydrogenase 3.5e-07
    HMMTigr Light-dependent protochlorophyllide reductase 1.4e-251
    HMMPfam Short-chain dehydrogenase/reductase SDR 1.7e-18
    HMMPanther PROTOCHLOROPHYLLIDE REDUCTASE 1.4e-181
    HMMPanther Short-chain dehydrogenase/reductase SDR 1.4e-181
    Gene3D NAD(P)-binding domain 7.2e-41
    superfamily NAD(P)-binding domain 1.3e-40

    See the detailed result >

    AT4G27440.1

    Model type
    Protein coding
    Short Description
    protochlorophyllide oxidoreductase B
    Curator Summary
    light-dependent NADPH:protochlorophyllide oxidoreductase B
    Computational Description
    protochlorophyllide oxidoreductase B (PORB); FUNCTIONS IN: oxidoreductase activity, protochlorophyllide reductase activity; INVOLVED IN: chlorophyll biosynthetic process, response to ethylene stimulus; LOCATED IN: chloroplast thylakoid membrane, chloroplast, membrane, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Light-dependent protochlorophyllide reductase (InterPro:IPR005979), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: protochlorophyllide oxidoreductase A (TAIR:AT5G54190.1); Has 19396 Blast hits to 19385 proteins in 2086 species: Archae - 175; Bacteria - 10514; Metazoa - 1820; Fungi - 1776; Plants - 1038; Viruses - 0; Other Eukaryotes - 4073 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR