F27248

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27248

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27248

Determination of introduced cDNA(s)

  • RAFL05-02-M17 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-02-M17 - Resource: pda00715

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-02-M17 - full reading 
    GTGTGAAAAA TTCTTCTTCT TCTTCTTCTC AGCGATTGAA TCGAACCAAG 
    AGCGAAAATG AGAAATTTCA GTGTCTTCGA GAGATTCTCT AAAGCTTTTA 
    AAGATCATCC TTCTCTCACC AGGATTCTCG TCGTCTCCAC GATCAGTGGT 
    GGTGGACTTA TAGCGTATTC TGAGGCAAAC GCATCTTATG GAGCAAATGG 
    TGGTGCTGTT GTTGAAACTG GAACCAAGAA GAAGAAGGTG GTGCTGCTCG 
    GAACTGGATG GGCTGGAACT AGTTTCTTGA AGAATTTGAA CAATTCTCAA 
    TATGAGGTTC AGATTATATC GCCTCGGAAC TACTTTGCTT TCACTCCTTT 
    ACTACCTAGT GTTACTTGTG GAACTGTTGA AGCTCGTAGT GTTGTTGAGC 
    CTATTCGAAA CATTGGAAGA AAGAATGTTG ATACTTCTTA CTTGGAGGCA 
    GAATGTTTCA AAATTGATCC AGCTAGCAAG AAGGTTTACT GCCGATCCAA 
    ACAAGGCCTT AGTTCAAATG GAAAGAAAGA ATTTTCTGTT GACTATGACT 
    ACCTTGTAAT TGCTACTGGA GCTCAGTCTA ACACCTTTAA CATACCCGGA 
    GTGGAGGAGA ACTGTCATTT TCTGAAAGAG GTTGAAGATG CTCAGAGAAT 
    CCGTAAAACT GTAATTGATT CCTTTGAGAA GGCAAGTCTA CCAGAACTAA 
    GCGATGAAGA GAGAAAGAGA ATCCTGCATT TTGTGGTTGT TGGTGGTGGG 
    CCAACAGGTG TTGAGTTTGC CGCTGAGCTA CATGATTTTG TTACTGAGGA 
    TCTAGTCAGT CTCTATCCTA GAGCCAAGGG TTCAGTGCGA ATCACGCTTC 
    TGGAGGCCGC AGACCACATC CTGACCATGT TTGACAAGAG AATCACTGAG 
    TTTGCCGAAG AAAAGTTTAG CAGAGATGGA ATTGATGTGA AACTTGGCTC 
    GATGGTAACT AAAGTGAATG AGAAAGATAT ATCTGCTAAA ACCAAAGGAG 
    GAGAGGTTTC CTCGATTCCT TATGGAATGA TTGTCTGGTC AACTGGTATT 
    GGAACTCGTC CTGTGATAAA AGATTTTATG AAACAAATTG GTCAGGGTAA 
    TAGACGAGCT CTGGCAACTG ATGAATGGCT TCGCGTAGAA GGAACTGATA 
    ATATATATGC CCTTGGTGAT TGCGCAACCA TTAACCAGCG CAAAGTCATG 
    GAAGATGTTT CTGCTATTTT TAGCAAAGCA GACAAAGACA AGTCTGGGAC 
    ACTGACTCTC AAAGAGTTCC AAGAAGCAAT GGATGACATT TGTGTTAGAT 
    ACCCTCAAGT GGAGCTCTAC TTGAAGAGTA AACGTATGCG TGGCATTGCA 
    GATCTTCTTA AAGAAGCCGA AACTGATGAT GTATCTAAGA ATAACATTGA 
    ACTGAAGATC GAAGAGTTCA AATCGGCTCT ATCTCAGGTT GACTCACAAG 
    TGAAGTTTCT TCCAGCTACT GCACAGGTTG CTGCGCAGCA AGGTGCTTAC 
    CTAGCTAAAT GCTTTGACCG TATGGAAGAG TGTGAGAAGA GTCCAGAAGG 
    TCCCATTAGG ATGAGAGGAG AAGGTCGTCA CCGTTTCCGC CCCTTCAGGT 
    ATAGGCATCT GGGACAATTT GCACCACTGG GAGGGGAGCA AACGGCAGCA 
    CAACTTCCAG GAGATTGGGT TTCTATTGGA CACAGCAGCC AGTGGCTCTG 
    GTACTCCGTC TACGCCAGTA AGCAAGTGAG CTGGCGTACG AGAGTGCTTG 
    TGGTTTCAGA TTGGATGAGG CGTTTCATCT TTGGTAGAGA TTCCAGTAGC 
    ATCTGATTTT CAACCTCCAA GGCTCCAATA CACATATCTC TCTCTCTCAC 
    GAGTTACACT AACTTTCTCA TGAGGAAAAA GTTTTGATTC ACTTTAAAAA 
    ATGGAAATCC AAATCGGCTT TTATATTTTG TAATAAAATA ATGACCACAA 
    GTAACGAGGA GAGATTGCCT CGTTTCGTTG GCCATATCGT TGCTGATCCC 
    GAGGATTGTA CTCTCTGTTT TTCTCTGCAA ATATTCTTCA GGTTGGCTTT 
    TTACCTTTAT ATCCTCTTTT TTTGTAAGCT TTTTCCTCAA ATTTTGTTTA 
    GTACGATGAC TAAAACTGAG TTTTGTTATT TCTTGCTCTC TGTTTTTCTC 
    CTTCAATATC ATGGTCTTTT TTTCTTTCC

    Gene models with high sequence identity

    1. AT4G05020.1 [+] show detail - NAD(P)H dehydrogenase B2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther NADH DEHYDROGENASE-RELATED 1.1e-278
    HMMPanther NADH DEHYDROGENASE-RELATED 1.1e-278
    PatternScan EF-Hand 1, calcium-binding site 0
    Gene3D no description 2.5e-13
    Gene3D no description 1.9e-23
    Coil coiled-coil 0
    ProfileScan EF-HAND 2 13.147
    HMMPfam FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.3e-38
    HMMPfam Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region 3.8e-13
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 2.9e-05
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 2.9e-05
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 2.9e-05
    superfamily FAD/NAD(P)-binding domain 4.9e-27
    superfamily EF-hand 3.9e-06
    HMMSmart Calcium-binding EF-hand 0.00039

    See the detailed result >

    AT4G05020.1

    Model type
    Protein coding
    Short Description
    NAD(P)H dehydrogenase B2
    Computational Description
    NAD(P)H dehydrogenase B2 (NDB2); FUNCTIONS IN: disulfide oxidoreductase activity, oxidoreductase activity, FAD binding; INVOLVED IN: oxidation reduction; LOCATED IN: mitochondrion, extrinsic to mitochondrial inner membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (InterPro:IPR013027), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region (InterPro:IPR001327), EF-HAND 2 (InterPro:IPR018249); BEST Arabidopsis thaliana protein match is: NAD(P)H dehydrogenase B3 (TAIR:AT4G21490.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR