F27429

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27429

Determination of introduced cDNA(s)

RAFL05-18-A12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-18-A12 - Resource: pda17118

full reading [+] show sequence

CCGGCTTTGT TACCGGAGCT CTGGACGGAG ATCCTTGTAC CGATTTGTGC 
GGTGATTGGT ATCGCCTTTT CGCTTTTCCA ATGGTACGTT GTATCTCGCG 
TGAAACTCAC CTCTGACCTC GGCGCATCGT CTTCCGGTGG AGCTAACAAT 
GGGAAGAATG GATACGGTGA TTATCTAATC GAGGAAGAGG AAGGTGTTAA 
TGACCAGAGT GTTGTCGCTA AGTGCGCTGA GATTCAGACT GCTATTTCCG 
AAGGTGCAAC TTCATTCCTA TTCACGGAGT ACAAATATGT TGGTGTCTTC 
ATGATTTTCT TTGCTGCTGT TATCTTTGTT TTCCTCGGCT CTGTTGAGGG 
ATTCAGCACT GATAACAAGC CTTGTACTTA CGACACCACC AGAACCTGCA 
AGCCTGCATT GGCTACTGCA GCTTTCAGTA CCATTGCTTT CGTGCTTGGT 
GCTGTTACCT CTGTTCTATC TGGTTTCCTT GGGATGAAGA TTGCTACATA 
CGCTAATGCT AGGACCACTT TGGAGGCGAG GAAAGGTGTT GGAAAGGCGT 
TCATTGTTGC ATTCAGGTCT GGTGCTGTGA TGGGTTTCCT TCTTGCAGCG 
AGTGGTCTAT TGGTGCTTTA CATTACTATC AATGTGTTCA AGATCTATTA 
CGGAGATGAC TGGGAAGGTC TTTTTGAGGC TATTACTGGT TATGGTCTTG 
GTGGGTCTTC CATGGCTCTC TTTGGCCGTG TTGGTGGTGG GATCTACACT 
AAGGTTGCTG ATGTCGGCGC TGACCTTGTC GGTAAAATTG AGAGGAATAT 
TCCAGAGGAT GATCCAAGAA ACCCAGCTGT CATTGCTGAT AATGTCGGTG 
ACAATGTTGG TGACATTGCT GGTATGGGAT CTGATCTCTT TGGATCATAT 
GCTGAAGCAT CATGCGCTGC TCTTGTTGTT GCCTCGATCT CATCTTTCGG 
AATCAACCAC GACTTCACTG CCATGTGCTA CCCATTGCTC ATCAGTTCAA 
TGGGAATCTT GGTTTGTTTG ATCACAACTC TCTTTGCCAC TGACTTCTTT 
GAGATTAAGC TTGTCAAGGA GATTGAACCA GCATTGAAGA ACCAGCTCAT 
TATCTCAACT GTTATTATGA CTGTTGGTAT TGCTATTGTG TCATGGGTTG 
GCTTACCGAC CTCCTTTACC ATCTTCAACT TTGGAACACA AAAAGTTGTC 
AAGAACTGGC AGCTATTCCT TTGTGTTTGT GTTGGTCTTT GGGCTGGACT 
CATTATTGGT TTCGTCACTG AGTACTACAC TAGTAACGCC TACAGCCCTG 
TGCAAGATGT TGCAGATTCA TGCAGAACTG GTGCAGCTAC CAATGTTATC 
TTCGGCCTTG CTCTTGGTTA CAAATCCGTC ATTATTCCAA TCTTTGCTAT 
TGCTATCAGT ATATTCGTTA GCTTCAGCTT TGCTGCTATG TATGGTGTTG 
CTGTTGCTGC TCTTGGTATG CTCAGTACCA TTGCCACTGG TTTGGCAATT 
GATGCTTATG GTCCCATCAG TGACAATGCT GGTGGTATTG CTGAAATGGC 
TGGAATGAGC CACCGCATCC GTGAAAGAAC TGATGCTCTT GATGCCGCTG 
GAAACACCAC TGCTGCTATT GGAAAGGGAT TTGCCATTGG CTCTGCTGCC 
CTAGTCTCCT TGGCTCTCTT TGGTGCCTTT GTGAGCCGTG CAGGGATCCA 
CACCGTAGAT GTTTTGACCC CTAAAGTTAT CATTGGGCTC CTTGTTGGTG 
CCATGCTTCC TTACTGGTTC TCTGCCATGA CAATGAAGAG TGTGGGAAGT 
GCAGCTCTTA AGATGGTTGA AGAAGTTCGC AGGCAGTTCA ACACCATCCC 
TGGACTTATG GAAGGAACCG CAAAACCAGA CTACGCCACA TGTGTCAAGA 
TCTCCACCGA TGCTTCCATC AAGGAAATGA TACCTCCTGG TTGCCTTGTC 
ATGCTCACAC CTCTCATTGT TGGTTTCTTC TTTGGAGTTG AGACCCTCTC 
TGGTGTCCTC GCCGGATCTC TTGTATCCGG TGTTCAGATC GCCATATCAG 
CATCTAACAC TGGTGGTGCC TGGGACAACG CCAAGAAATA CATCGAGGCT 
GGTGTATCAG AGCACGCAAA GAGCCTTGGA CCAAAGGGTT CAGAGCCACA 
CAAGGCAGCT GTGATTGGAG ACACAATTGG AGACCCATTG AAGGATACTT 
CAGGACCTTC ATTGAACATC CTCATCAAGC TCATGGCTGT TGAGTCTCTT 
GTCTTTGCTC CCTTCTTCGC CACTCACGGT GGTATCCTTT TCAAGTACTT 
CTAAACTCAA TCCGAGGGAA GAAGATGACG ATGATGAAGA AGAAGAAGAT 
GATGATGGCG ATCGATTCTA AACTTTCTTT TTTACCATTC TTATTTTCGT 
TTACCGTAGG TGGTTAAAAA ACCTTTTTTG TTGATGAGGC TCATTTAAAG 
AACCAACCAA ATGATGTTTC TTTCTCTCAC TCTCTGTCTT TCTGTTTTCT 
TTTTGTTCTG TTTAGAATTT AGAAATCCAC CAAGTATTCG GTCGAGACTT 
GTTTTAGCCG TTACTTTCTG CTGCTTATAT TTCCTAAATT GGTTGTCTTC 
TTCGAGACAT AATTGGAATC AAAAAAAAAA

Gene models with high sequence identity

AT1G15690.1 [+] show detail - Inorganic H pyrophosphatase family protein
E-value: 0; Score: 99.96

InterPro Scan Digest

Program	Description	E-value
HMMTigr	Inorganic H+ pyrophosphatase	0
HMMPIR	Inorganic H+ pyrophosphatase	0
HMMPfam	Inorganic H+ pyrophosphatase	0

See the detailed result >

AT1G15690.1

Model type: Protein coding
Short Description: Inorganic H pyrophosphatase family protein
Curator Summary: Encodes a H(+)-translocating (pyrophosphate-energized) inorganic pyrophosphatase (H(+)-PPase; EC 3.6.1.1) located in the vacuolar membrane. Expression is found in all tissues examined, including meristems and floral organ primordium. Expression is particularly enhanced in pollen, and is repressed by light. Over expression and loss of function phenotypes suggest AVP1 is involved in regulation of apoplastic pH and auxin transport. The effect on auxin transport likely involves effects of extracellular pH on subcellular localization of auxin efflux carriers such as PIN1.
Computational Description: AVP1; FUNCTIONS IN: hydrogen-translocating pyrophosphatase activity; INVOLVED IN: response to water deprivation, response to salt stress, establishment or maintenance of transmembrane electrochemical gradient, leaf development, auxin polar transport; LOCATED IN: in 10 components; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Inorganic H+ pyrophosphatase (InterPro:IPR004131); BEST Arabidopsis thaliana protein match is: Inorganic H pyrophosphatase family protein (TAIR:AT1G16780.1); Has 6470 Blast hits to 6448 proteins in 793 species: Archae - 67; Bacteria - 1322; Metazoa - 5; Fungi - 1; Plants - 304; Viruses - 0; Other Eukaryotes - 4771 (source: NCBI BLink).
Link: InterPro Scan - TAIR