F27443

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27443

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
T2  
whole plant (PO:0000003)
  • increased height (PATO:0000570)
    (Adult plant::Plant height::tall)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Other)
  • abnormal (PATO:0000460)
    (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27443

Determination of introduced cDNA(s)

  • RAFL09-95-H16 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-95-H16 - Resource: pda09093

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-95-H16 - full reading 
    ACGCTTCCGA TCTCTAAAAC CTGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
    GACAGAGAGA AGTGTTTCAA TAGTTTGGCG TGAAGATGGC CGGAGGAGGC 
    GGATACGGCG GCGCATCGGG GAAAGTTGAT TACGTCTTCA AAGTTGTTCT 
    AATCGGAGAT TCGGCTGTTG GGAAATCGCA ACTACTTGCT CGATTCGCTA 
    GAGACGAATT CAGCATGGAT TCTAAAGCCA CCATCGGCGT CGAGTTCCAA 
    ACTCGTACCC TCTCCATTGA ACAAAAAAGC ATTAAGGCTC AGATCTGGGA 
    TACCGCTGGT CAGGAAAGAT ACAGAGCCGT TACAAGCGCA TACTACAGAG 
    GAGCAGTTGG CGCAATGCTG GTTTATGATA TGACGAAACG TGAGACCTTT 
    GAGCATATTC CGCGTTGGCT TGAAGAACTG AGGGCGCACG CTGATAAGAA 
    CATTGTCATC ATCCTTATTG GAAACAAGTC TGATCTAGAA GATCAAAGAG 
    CTGTTCCCAC TGAAGACGCT AAAGAGTTTG CTGAGAAGGA AGGACTCTTT 
    TTCCTCGAGA CCTCTGCTTT AAACGCAACC AATGTCGAAA ACTCCTTCAA 
    CACTCTAATG ACTCAGATAT ACAATACCGT GAACAAGAAG AATCTTGCAT 
    CTGAAGGCGA CTCAAATAAC CCCGGTTCAT TGGCTGGTAA GAAGATTCTC 
    ATCCCAGGTT CTGGACAGGA GATTCCCGCT AAGACCAGCA CTTGTTGTAC 
    TTCTTCTTGA TCTGTCTCCT ACTCAAGCAA GATTCATTTT TTTTCCTCCT 
    GAAATTTGTG ATAGAGAATG CTACTTTCAT TGTATATTCT TTTTCGAATC 
    TGGCTTGTTT GCTAGTTCAT TAAACATTGG TGTGCAATTG GACACCGATA 
    GAAAGTATTG GAACAAATAG CTTTTGAATG AAATATGAAA CAAACAGTCT 
    TTGAGAGT

    Gene models with high sequence identity

    1. AT4G39990.1 [+] show detail - RAB GTPase homolog A4B
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily P-loop containing nucleoside triphosphate hyd 7.7e-62
    FPrintScan Ras GTPase 4.1e-40
    FPrintScan Ras GTPase 4.1e-40
    FPrintScan Ras GTPase 4.1e-40
    FPrintScan Ras GTPase 4.1e-40
    FPrintScan Ras GTPase 4.1e-40
    HMMTigr Small GTP-binding protein 1.3e-34
    ProfileScan Small GTPase 36.037
    HMMPfam Ras 8.5e-62
    HMMPanther Rab11-related 2.7e-95
    HMMPanther RAS-RELATED GTPASE 2.7e-95
    Gene3D no description 1.1e-61
    HMMSmart ADP-ribosylation factor 0.0033
    HMMSmart Ras small GTPase, Ras type 9.7e-23
    HMMSmart Ras small GTPase, Rab type 1.8e-105
    HMMSmart Small GTPase, Rho type 0.0000000015
    HMMSmart Ran GTPase 0.00000017

    See the detailed result >

    AT4G39990.1

    Model type
    Protein coding
    Short Description
    RAB GTPase homolog A4B
    Curator Summary
    GTP-binding protein ATGB3
    Computational Description
    RAB GTPase homolog A4B (RABA4B); FUNCTIONS IN: GTP binding; INVOLVED IN: endocytic recycling; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ras GTPase (InterPro:IPR001806), Small GTP-binding protein (InterPro:IPR005225), Small GTPase (InterPro:IPR020851), Ras (InterPro:IPR013753), Ras small GTPase, Rab type (InterPro:IPR003579), Rab11-related (InterPro:IPR015595); BEST Arabidopsis thaliana protein match is: RAB GTPase homolog A4A (TAIR:AT5G65270.1); Has 29206 Blast hits to 29157 proteins in 775 species: Archae - 30; Bacteria - 156; Metazoa - 15160; Fungi - 4303; Plants - 3469; Viruses - 20; Other Eukaryotes - 6068 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR