F27443

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) decreased size (PATO:0000587) (Rosette leaf::Shape::small) increased width (PATO:0000600) (Rosette leaf::Shape::wide)
	stem (PO:0009047)	increased thickness (PATO:0000591) (Stem::Shape::thick)
T2
	whole plant (PO:0000003)	increased height (PATO:0000570) (Adult plant::Plant height::tall)
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small) dark green (PATO:0001249) (Cauline leaf::Color::dark)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) increased width (PATO:0000600) (Rosette leaf::Shape::wide)
	fruit (PO:0009001)	abnormal (PATO:0000460) (Silique::Other) abnormal (PATO:0000460) (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27443

Determination of introduced cDNA(s)

RAFL09-95-H16 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-95-H16 - Resource: pda09093

full reading [+] show sequence

ACGCTTCCGA TCTCTAAAAC CTGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
GACAGAGAGA AGTGTTTCAA TAGTTTGGCG TGAAGATGGC CGGAGGAGGC 
GGATACGGCG GCGCATCGGG GAAAGTTGAT TACGTCTTCA AAGTTGTTCT 
AATCGGAGAT TCGGCTGTTG GGAAATCGCA ACTACTTGCT CGATTCGCTA 
GAGACGAATT CAGCATGGAT TCTAAAGCCA CCATCGGCGT CGAGTTCCAA 
ACTCGTACCC TCTCCATTGA ACAAAAAAGC ATTAAGGCTC AGATCTGGGA 
TACCGCTGGT CAGGAAAGAT ACAGAGCCGT TACAAGCGCA TACTACAGAG 
GAGCAGTTGG CGCAATGCTG GTTTATGATA TGACGAAACG TGAGACCTTT 
GAGCATATTC CGCGTTGGCT TGAAGAACTG AGGGCGCACG CTGATAAGAA 
CATTGTCATC ATCCTTATTG GAAACAAGTC TGATCTAGAA GATCAAAGAG 
CTGTTCCCAC TGAAGACGCT AAAGAGTTTG CTGAGAAGGA AGGACTCTTT 
TTCCTCGAGA CCTCTGCTTT AAACGCAACC AATGTCGAAA ACTCCTTCAA 
CACTCTAATG ACTCAGATAT ACAATACCGT GAACAAGAAG AATCTTGCAT 
CTGAAGGCGA CTCAAATAAC CCCGGTTCAT TGGCTGGTAA GAAGATTCTC 
ATCCCAGGTT CTGGACAGGA GATTCCCGCT AAGACCAGCA CTTGTTGTAC 
TTCTTCTTGA TCTGTCTCCT ACTCAAGCAA GATTCATTTT TTTTCCTCCT 
GAAATTTGTG ATAGAGAATG CTACTTTCAT TGTATATTCT TTTTCGAATC 
TGGCTTGTTT GCTAGTTCAT TAAACATTGG TGTGCAATTG GACACCGATA 
GAAAGTATTG GAACAAATAG CTTTTGAATG AAATATGAAA CAAACAGTCT 
TTGAGAGT

Gene models with high sequence identity

AT4G39990.1 [+] show detail - RAB GTPase homolog A4B
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
superfamily	P-loop containing nucleoside triphosphate hyd	7.7e-62
FPrintScan	Ras GTPase	4.1e-40
FPrintScan	Ras GTPase	4.1e-40
FPrintScan	Ras GTPase	4.1e-40
FPrintScan	Ras GTPase	4.1e-40
FPrintScan	Ras GTPase	4.1e-40
HMMTigr	Small GTP-binding protein	1.3e-34
ProfileScan	Small GTPase	36.037
HMMPfam	Ras	8.5e-62
HMMPanther	Rab11-related	2.7e-95
HMMPanther	RAS-RELATED GTPASE	2.7e-95
Gene3D	no description	1.1e-61
HMMSmart	ADP-ribosylation factor	0.0033
HMMSmart	Ras small GTPase, Ras type	9.7e-23
HMMSmart	Ras small GTPase, Rab type	1.8e-105
HMMSmart	Small GTPase, Rho type	1.5e-09
HMMSmart	Ran GTPase	1.7e-07

See the detailed result >

AT4G39990.1

Model type: Protein coding
Short Description: RAB GTPase homolog A4B
Curator Summary: GTP-binding protein ATGB3
Computational Description: RAB GTPase homolog A4B (RABA4B); FUNCTIONS IN: GTP binding; INVOLVED IN: endocytic recycling; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ras GTPase (InterPro:IPR001806), Small GTP-binding protein (InterPro:IPR005225), Small GTPase (InterPro:IPR020851), Ras (InterPro:IPR013753), Ras small GTPase, Rab type (InterPro:IPR003579), Rab11-related (InterPro:IPR015595); BEST Arabidopsis thaliana protein match is: RAB GTPase homolog A4A (TAIR:AT5G65270.1); Has 29206 Blast hits to 29157 proteins in 775 species: Archae - 30; Bacteria - 156; Metazoa - 15160; Fungi - 4303; Plants - 3469; Viruses - 20; Other Eukaryotes - 6068 (source: NCBI BLink).
Link: InterPro Scan - TAIR