F27519

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27519

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27519

Determination of introduced cDNA(s)

  • RAFL05-02-N23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-02-N23 - Resource: pda07233

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-02-N23 - full reading 
    ACACTCCTTA TCTTCTTCAT TCCTCCATCC TATTTTTCTT CTTCGCCAAA 
    GTGTTATTCC TCAACTTTAT ATATTACACA AATGTCTTTA GCCGCGGATA 
    ATGTGTTGTT GGTGGAAGAA GGAAGGCCAG CCACAGCGGA ACATCCATCG 
    GCCGGACCGG TTTATCGATG TAAATACGCT AAAGATGGCC TCCTCGATCT 
    CCCTACCGAT ATTGATTCTC CTTGGCAGTT CTTTAGTGAG GCTGTGAAGA 
    AATATCCGAA TGAGCAAATG TTGGGCCAAC GCGTAACGAC TGATTCTAAG 
    GTCGGTCCAT ACACGTGGAT CACATATAAG GAAGCGCACG ACGCTGCAAT 
    TCGGATTGGA TCAGCAATCA GAAGCCGAGG CGTTGATCCG GGACACTGTT 
    GTGGTATTTA CGGAGCTAAT TGTCCAGAAT GGATTATTGC AATGGAGGCC 
    TGCATGAGCC AAGGGATCAC CTACGTGCCT CTATACGATT CTTTAGGCGT 
    AAACGCAGTT GAATTCATCA TCAACCACGC CGAGGTTTCG CTAGTATTTG 
    TTCAAGAGAA GACAGTTTCA TCGATCTTAT CGTGCCAAAA GGGATGTTCT 
    TCGAATTTGA AGACTATTGT GAGCTTCGGG GAAGTCTCGA GTACACAAAA 
    GGAAGAAGCT AAGAACCAAT GTGTTTCTTT ATTTTCATGG AATGAGTTCT 
    CACTAATGGG AAACTTAGAT GAGGCAAATC TACCTCGTAA GCGAAAGACA 
    GACATCTGCA CAATAATGTA CACAAGCGGG ACGACTGGAG AACCCAAAGG 
    TGTAATCTTA AACAACGCAG CAATTTCGGT CCAGGTTTTA TCCATAGACA 
    AAATGCTTGA AGTCACTGAT CGATCGTGTG ACACGAGCGA TGTGTTCTTC 
    TCGTACTTGC CATTAGCACA TTGCTATGAT CAAGTCATGG AGATTTACTT 
    TTTATCTAGA GGCTCCTCTG TTGGATACTG GCGTGGCGAC ATTCGGTACC 
    TGATGGATGA TGTTCAAGCT CTTAAACCTA CTGTGTTTTG CGGTGTTCCA 
    CGAGTTTACG ACAAACTATA TGCCGGTATA ATGCAAAAAA TATCAGCTAG 
    TGGCTTGATA CGCAAGAAAC TGTTTGATTT TGCTTATAAC TACAAATTGG 
    GAAATATGAG AAAAGGATTC TCTCAAGAAG AAGCTTCTCC TCGTCTAGAC 
    AGACTTATGT TCGATAAGAT AAAAGAAGCA TTAGGAGGAA GAGCTCATAT 
    GTTGTTATCA GGAGCAGCGC CTCTACCTCG TCATGTAGAG GAGTTCTTGA 
    GAATCATTCC TGCCTCTAAT CTCTCTCAAG GTTATGGATT GACTGAGAGT 
    TGTGGGGGAA GCTTCACGAC CTTAGCCGGA GTATTTTCTA TGGTGGGGAC 
    AGTGGGTGTG CCAATGCCCA CGGTGGAGGC AAGGCTAGTG TCCGTACCAG 
    AGATGGGTTA CGACGCCTTT TCCGCTGACG TGCCGAGAGG AGAGATTTGT 
    CTTAGAGGAA ATTCAATGTT TTCTGGTTAC CATAAAAGAC AAGATCTAAC 
    TGATCAAGTC CTAATCGATG GATGGTTCCA CACAGGAGAT ATTGGAGAAT 
    GGCAAGAAGA TGGATCAATG AAGATCATCG ATAGGAAGAA GAACATCTTC 
    AAGTTGTCTC AAGGTGAATA TGTTGCTGTT GAAAACCTCG AAAACACTTA 
    CTCAAGATGT CCCCTCATTG CTCAGATATG GGTCTATGGC AACAGCTTCG 
    AGTCATTTTT GGTAGGTGTG GTTGTACCTG ATAGAAAAGC TATTGAAGAT 
    TGGGCTAAAC TCAATTACCA ATCTCCCAAT GATTTCGAAT CTCTATGTCA 
    AAATCTCAAA GCTCAAAAAT ACTTCTTGGA TGAGCTTAAC TCTACCGCAA 
    AGCAATATCA ACTTAAAGGA TTTGAAATGT TAAAAGCTAT TCATTTAGAA 
    CCAAACCCTT TTGATATTGA AAGAGATCTT ATTACTCCAA CTTTCAAGCT 
    GAAAAGGCCA CAGCTCCTCC AACATTACAA GGGCATAGTT GATCAACTTT 
    ATTCAGAAGC AAAGAGGTCC ATGGCATAGA TAATTAGATA CCGATCGGCT 
    ATTCATTTTC TGATGCTATT TGGGTTTGTA TGATATGTTA CAAAATTGTG 
    TTCGGATCCT CTCATCATGT GATTCTTGTT ACACTAAATT TATAGTTTGG 
    AAACTATATA TATACTATTA AGCAACGTTT ACG

    Gene models with high sequence identity

    1. AT1G49430.1 [+] show detail - long-chain acyl-CoA synthetase 2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam AMP-dependent synthetase/ligase 3.1e-102
    Gene3D no description 5.6e-30
    Gene3D no description 1.8e-05
    Gene3D no description 3.5e-17
    PatternScan AMP-binding, conserved site 0
    HMMPanther LONG-CHAIN-FATTY-ACID COA LIGASE 6.3e-289
    HMMPanther ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBE 6.3e-289
    superfamily Acetyl-CoA synthetase-like 5.9e-117

    See the detailed result >

    AT1G49430.1

    Model type
    Protein coding
    Short Description
    long-chain acyl-CoA synthetase 2
    Curator Summary
    Encodes a long chain acyl-CoA synthetase that catalyzes the synthesis of omega-hydroxy fatty acyl-CoA intermediates in the pathway to cutin synthesis. Required for repression of lateral root formation.
    Computational Description
    long-chain acyl-CoA synthetase 2 (LACS2); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: AMP-dependent synthetase and ligase family protein (TAIR:AT1G64400.1); Has 53337 Blast hits to 50359 proteins in 3209 species: Archae - 917; Bacteria - 34261; Metazoa - 2388; Fungi - 2032; Plants - 1855; Viruses - 1; Other Eukaryotes - 11883 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR