F27631

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27631

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27631

Determination of introduced cDNA(s)

  • RAFL05-16-E22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-16-E22 - Resource: pda17098

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-16-E22 - full reading 
    CCTGGTGCGC TCTCATATTT CTCACATTTT CGTAGCCGCA AGACTCCTTT 
    CAGATTCTTA CTTGCAGCTA TGGGTAAAGA GAAGTTTCAC ATTAACATTG 
    TGGTCATTGG TCATGTTGAT TCTGGAAAAT CGACCACAAC TGGTCACTTG 
    ATCTATAAGC TTGGTGGTAT TGACAAGCGT GTCATCGAGA GGTTCGAGAA 
    GGAGGCTGCT GAGATGAACA AGAGGTCCTT CAAGTACGCA TGGGTGTTGG 
    ACAAACTTAA GGCCGAGCGT GAGCGTGGTA TTACCATCGA TATTGCTCTA 
    TGGAAGTTCG AGACCACCAA GTACTACTGC ACAGTCATTG ATGCCCCAGG 
    ACATCGTGAT TTCATCAAGA ACATGATTAC TGGTACCTCC CAGGCTGATT 
    GTGCTGTTCT TATCATTGAC TCCACCACTG GAGGTTTTGA GGCTGGTATC 
    TCTAAGGATG GTCAGACCCG TGAGCACGCT CTTCTTGCTT TCACCCTTGG 
    TGTCAAGCAG ATGATTTGCT GTTGTAACAA GATGGATGCC ACCACCCCCA 
    AATACTCCAA GGCTAGGTAC GATGAAATCA TCAAGGAGGT GTCTTCATAC 
    CTGAAGAAGG TCGGATACAA CCCTGACAAA ATCCCATTTG TGCCAATCTC 
    TGGATTCGAG GGAGACAACA TGATTGAGAG GTCAACCAAC CTTGACTGGT 
    ACAAGGGACC AACTCTTCTT GAGGCTCTTG ACCAGATCAA CGAGCCCAAG 
    AGGCCATCAG ACAAGCCCCT TCGTCTTCCA CTTCAGGATG TCTACAAGAT 
    TGGTGGTATT GGAACGGTGC CAGTGGGACG TGTTGAGACT GGTATGATCA 
    AGCCTGGTAT GGTTGTTACC TTTGCTCCCA CAGGGTTGAC CACTGAGGTT 
    AAGTCTGTTG AGATGCACCA CGAGTCTCTT CTTGAGGCAC TTCCCGGTGA 
    CAATGTTGGA TTCAATGTCA AGAATGTTGC TGTCAAGGAT CTTAAGAGAG 
    GATACGTTGC CTCTAACTCC AAGGATGATC CAGCTAAGGG TGCCGCCAAC 
    TTCACCTCCC AGGTCATCAT CATGAACCAC CCTGGTCAGA TTGGTAACGG 
    TTACGCCCCA GTTCTCGATT GCCACACCTC TCACATTGCA GTCAAGTTCT 
    CTGAGATCTT GACCAAGATT GACAGGCGTT CTGGTAAGGA GATTGAGAAG 
    GAGCCCAAGT TTTTGAAGAA TGGTGACGCT GGTATGGTTA AGATGACCCC 
    AACCAAGCCC ATGGTTGTTG AGACTTTCTC CGAGTACCCA CCTTTGGGAC 
    GTTTCGCTGT TAGGGACATG AGGCAGACCG TTGCTGTTGG TGTTATTAAG 
    AGCGTGGACA AGAAGGACCC AACTGGAGCC AAGGTCACCA AGGCTGCAGT 
    GAAGAAGGGT GCCAAATGAT GAGACTTTCG TTATGATCGA CTCTCTTATG 
    GTTTTCTTTG GTTCTTAAAA CTTTGATGGC GTTTGAGCCT TTTTCTTTTT 
    TCTCTTTATT TCTGTGACTT TCTCTCTCCC TCCTTTTTGG ATATCTCTGA 
    GACTTTTTAT TATGGTTTTC AATTATGCAG TTTCCGGATA ATTTTGCTTC 
    AAAAAAAAAA

    Gene models with high sequence identity

    1. AT5G60390.1 [+] show detail - GTP binding Elongation factor Tu family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Protein synthesis factor, GTP-binding 1.8e-23
    FPrintScan Protein synthesis factor, GTP-binding 1.8e-23
    FPrintScan Protein synthesis factor, GTP-binding 1.8e-23
    FPrintScan Protein synthesis factor, GTP-binding 1.8e-23
    FPrintScan Protein synthesis factor, GTP-binding 1.8e-23
    HMMPfam Protein synthesis factor, GTP-binding 2.5e-55
    HMMPfam Translation elongation factor EFTu/EF1A, C-terminal 1.6e-32
    HMMPfam Translation elongation factor EFTu/EF1A, domain 2 6.9e-16
    HMMPanther ELONGATION FACTOR 1-ALPHA (EF-1-ALPHA) 0
    HMMPanther TRANSLATION FACTOR 0
    BlastProDom Q46515_DESMO_Q46515; 2e-06
    superfamily P-loop containing nucleoside triphosphate hyd 2.6e-84
    superfamily Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal 2.4e-41
    superfamily Translation elongation/initiation factor/Ribosomal, beta-barrel 1.6e-32
    PatternScan Protein synthesis factor, GTP-binding 0
    HMMTigr Translation elongation factor EF1A, eukaryotic/archaeal 7.4e-292
    Gene3D no description 8.1e-58
    Gene3D no description 9.6e-31
    Gene3D no description 3.5e-45

    See the detailed result >

    AT5G60390.1

    Model type
    Protein coding
    Short Description
    GTP binding Elongation factor Tu family protein
    Computational Description
    GTP binding Elongation factor Tu family protein; FUNCTIONS IN: calmodulin binding, translation elongation factor activity; INVOLVED IN: translational elongation; LOCATED IN: mitochondrion, nucleus, plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, seed development stages; CONTAINS InterPro DOMAIN/s: Translation elongation factor EFTu/EF1A, C-terminal (InterPro:IPR004160), Translation elongation factor EFTu/EF1A, domain 2 (InterPro:IPR004161), Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal (InterPro:IPR009001), Protein synthesis factor, GTP-binding (InterPro:IPR000795), Translation elongation/initiation factor/Ribosomal, beta-barrel (InterPro:IPR009000), Translation elongation factor EF1A, eukaryotic/archaeal (InterPro:IPR004539); BEST Arabidopsis thaliana protein match is: GTP binding Elongation factor Tu family protein (TAIR:AT1G07940.2); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR