F28016

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28016

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
T2  
cauline leaf (PO:0000013)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28016

Determination of introduced cDNA(s)

  • RAFL09-10-L23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-10-L23 - Resource: pda05379

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-10-L23 - full reading 
    TGGCAACACA AAAACATACA CACTACTAAT AAACCATGAA GCTTCTTATG 
    CTCGCCTTTG TTTTTCTATT AGCTTTGGCG ACTTGTAAAG GTGACGAGTT 
    TGTTTGTGAA GAGAACGAGC CATTCACATG TAACCAAACT AAACTTTTCA 
    ACAGTGGCAA TTTCGAAAAA GGCTTCATCT TCGGTGTTGC ATCTTCTGCT 
    TACCAGGTGG AAGGCGGTAG AGGCCGTGGA CTTAACGTTT GGGATAGCTT 
    CACTCACCGA TTCCCAGAGA AAGGTGGAGC TGATTTGGGA AATGGAGACA 
    CTACTTGTGA CTCATATACT CTTTGGCAGA AAGATATAGA CGTGATGGAC 
    GAGCTCAACT CTACTGGCTA CAGATTCTCC ATTGCGTGGT CAAGACTCCT 
    TCCAAAAGGA AAGAGGAGCA GGGGAGTGAA CCCAGGAGCT ATTAAGTACT 
    ACAACGGTCT CATAGATGGC CTCGTCGCAA AGAATATGAC GCCCTTTGTT 
    ACCCTCTTTC ATTGGGACCT TCCTCAAACA CTACAAGATG AATATAACGG 
    TTTCTTGAAC AAAACGATCG TAGACGATTT CAAGGATTAC GCGGATCTAT 
    GTTTCGAGTT ATTTGGTGAT AGGGTAAAGA ACTGGATCAC CATCAACCAG 
    CTATACACAG TGCCTACTAG AGGATATGCA TTGGGAACAG ATGCACCCGG 
    TCGATGTTCT CCTAAGATTG ACGTTAGATG TCCCGGCGGA AATTCGTCAA 
    CAGAACCCTA TATTGTTGCA CATAACCAGC TTCTTGCTCA TGCAGCGGCC 
    GTTGATGTTT ACAGGACGAA ATATAAGGAT GACCAAAAAG GTATGATTGG 
    ACCAGTGATG ATAACTAGAT GGTTTCTTCC ATTTGATCAT AGTCAAGAGA 
    GCAAAGATGC AACTGAGCGG GCTAAAATAT TTTTCCATGG ATGGTTCATG 
    GGGCCTCTAA CAGAAGGTAA ATACCCAGAC ATCATGAGGG AATATGTTGG 
    TGATCGGCTT CCAGAGTTCA GTGAAACAGA AGCCGCACTT GTAAAGGGTT 
    CATATGATTT TCTTGGTCTC AACTATTACG TCACTCAATA CGCCCAAAAT 
    AATCAGACGA TTGTTCCTTC GGACGTACAC ACTGCCTTGA TGGACTCACG 
    CACAACTCTC ACATCTAAAA ATGCAACTGG TCATGCTCCT GGTCCACCGT 
    TCAATGCAGC CAGTTACTAC TACCCAAAAG GCATTTACTA CGTAATGGAT 
    TACTTCAAAA CCACTTACGG TGACCCTTTA ATATATGTCA CTGAGAATGG 
    ATTTAGTACC GCAGGTGATG AGGACTTTGA GAAGGCTACT GCCGATTACA 
    AGCGGATTGA TTATCTCTGT AGTCATCTCT GTTTCCTCAG TAAAGTCATC 
    AAGGAGAAGA ATGTCAACGT GAAAGGATAT TTTGCTTGGT CTCTTGGGGA 
    TAATTACGAA TTCTGTAACG GATTTACCGT CAGATTCGGA CTAAGTTACG 
    TTGATTTCGC AAATATCACT GGTGATAGAG ACCTCAAAGC ATCTGGCAAA 
    TGGTTCCAGA AGTTCATAAA CGTTACCGAC GAAGACTCTA CGAACCAAGA 
    TCTACTCCGC TCAAGCGTCT CCTCCAAGAA CCGTGATCGG AAGAGTCTTG 
    CAGATGCATG AAATATCCAA TCCACTATAT GTCCACCAAG ATCATCTTCA 
    TGTTTCCTCT TTCTACTTGC TCCATAGATA AGGAGCTTTT TCTACCATAT 
    GTATTAAAAT AAAATCCTAA TAAAAGATGA TCAATAATAA TAAAGACTTT 
    GTTAT

    Gene models with high sequence identity

    1. AT5G26000.1 [+] show detail - thioglucoside glucohydrolase 1
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Glycoside hydrolase, family 1, active site 0
    PatternScan Glycoside hydrolase, family 1, active site 0
    superfamily Glycoside hydrolase, catalytic core 2.9e-166
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    Gene3D Glycoside hydrolase, subgroup, catalytic core 7.9e-168
    HMMPfam Glycoside hydrolase, family 1 4.6e-156
    HMMPanther BETA-GLUCOSIDASE 6.2e-286
    HMMPanther Glycoside hydrolase, family 1 6.2e-286

    See the detailed result >

    AT5G26000.1

    Model type
    Protein coding
    Short Description
    thioglucoside glucohydrolase 1
    Curator Summary
    member of Glycoside Hydrolase Family 1. encodes one of two known functional myrosinase enzymes in Arabidopsis. The enzyme catalyzes the hydrolysis of glucosinolates into compounds that are toxic to various microbes and herbivores.
    Computational Description
    thioglucoside glucohydrolase 1 (TGG1); FUNCTIONS IN: thioglucosidase activity, beta-glucosidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: glucosinolate catabolic process; LOCATED IN: in 7 components; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 1 (InterPro:IPR001360), Glycoside hydrolase, family 1, active site (InterPro:IPR018120), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: glucoside glucohydrolase 2 (TAIR:AT5G25980.2); Has 11138 Blast hits to 10810 proteins in 1458 species: Archae - 132; Bacteria - 7708; Metazoa - 712; Fungi - 191; Plants - 1422; Viruses - 0; Other Eukaryotes - 973 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR