F28245

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28245

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28245

Determination of introduced cDNA(s)

  • RAFL05-12-I22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-12-I22 - Resource: pda01218

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-12-I22 - full reading 
    ATGAACCAAA GTAAAAAACA AAGACTCGAG TTGATATGTC TAGTACTAAA 
    GATATGTCAA CTGTCCAAAA CGCAACTCCT TTTAACGGCG TTGCCCCATC 
    CACCACCGTG CGAGTTACAA TCGTCCAATC CTCCACCGTC TATAACGATA 
    CTCCTGCCAC TATAGACAAA GCGGAGAAGT ATATTGTGGA GGCGGCAAGC 
    AAGGGAGCAG AGCTAGTGTT GTTCCCGGAG GGGTTTATCG GTGGCTATCC 
    TCGAGGTTTT AGGTTCGGTT TAGCGGTTGG CGTTCATAAC GAAGAAGGGC 
    GTGATGAGTT TCGGAAGTAC CATGCTTCTG CTATTCATGT TCCTGGCCCT 
    GAAGTAGCAA GATTGGCTGA CGTGGCTAGG AAAAACCATG TGTACTTGGT 
    AATGGGAGCC ATAGAGAAGG AAGGGTATAC CCTCTATTGC ACAGTTCTTT 
    TCTTTAGTCC ACAGGGTCAG TTCTTGGGCA AGCACCGTAA ACTCATGCCC 
    ACAAGTTTGG AACGTTGCAT TTGGGGCCAA GGGGACGGAT CAACCATCCC 
    CGTTTACGAC ACTCCCATTG GAAAACTCGG TGCTGCTATT TGCTGGGAGA 
    ATAGGATGCC CCTCTACAGA ACTGCATTGT ACGCCAAAGG CATTGAGCTT 
    TATTGTGCAC CTACTGCTGA TGGTTCGAAA GAATGGCAAT CGTCGATGCT 
    TCACATTGCG ATCGAAGGTG GATGTTTCGT CTTGTCGGCT TGCCAATTCT 
    GCCAGCGTAA ACATTTCCCT GATCATCCTG ACTACTTGTT TACCGATTGG 
    TACGACGACA AAGAACATGA TTCTATTGTC TCCCAAGGTG GAAGTGTCAT 
    TATTTCACCT TTGGGACAAG TTCTCGCCGG ACCAAACTTT GAATCAGAGG 
    GTCTCGTCAC AGCTGATATT GATCTTGGTG ATATAGCAAG AGCCAAGTTA 
    TACTTCGATT CGGTTGGACA TTACTCGAGA CCAGATGTTT TACACTTGAC 
    CGTAAATGAG CACCCGAGGA AATCGGTTAC ATTCGTGACG AAGGTGGAGA 
    AAGCTGAGGA TGACTCAAAC AAATAGTAAG AGACTTGAAG TTCGTATCTG 
    CTGGAGTTAT GTCAATCGTA TGGAGTCAAG TCCAAAATGT TCTGTTGCGT 
    TTTCATTTTA TGTTCAAGTT TATTTATCTT TCTCTTCAAT GGTAAGATCT 
    ATGGAGTCAA GTAATAATGG TAAGACTTAT GTTGTTGAAT AAAACGAATT 
    GGTTCTCT

    Gene models with high sequence identity

    1. AT3G44310.1 [+] show detail - nitrilase 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 45.072
    PatternScan Nitrilase/cyanide hydratase, conserved site 0
    PatternScan Nitrilase/cyanide hydratase, conserved site 0
    HMMPfam Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 1.3e-41
    HMMPanther NITRILASE-RELATED 3.6e-73
    Gene3D Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 4.9e-71
    superfamily Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 1.6e-77

    See the detailed result >

    AT3G44310.1

    Model type
    Protein coding
    Short Description
    nitrilase 1
    Curator Summary
    Mutants are resistant to indole-3-acetonitrile (IAN). NIT1 catalyzes the terminal activation step in indole-acetic acid biosynthesis. Predominantly expressed isoform of nitrilase isoenzyme family. Aggregation of NIT1 in cells directly abutting wound sites is one of the earliest events associated with wound and herbicide-induced cell death. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide. It is also involved in the conversion of IAN to IAM (indole-3-acetamide) and other non-auxin-related metabolic processes.
    Computational Description
    nitrilase 1 (NIT1); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: indoleacetic acid biosynthetic process; LOCATED IN: apoplast, chloroplast, plasma membrane; EXPRESSED IN: guard cell, cultured cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 2 (TAIR:AT3G44300.1); Has 7572 Blast hits to 7496 proteins in 1731 species: Archae - 139; Bacteria - 4902; Metazoa - 443; Fungi - 562; Plants - 319; Viruses - 11; Other Eukaryotes - 1196 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR