F28816

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28816

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28816

Determination of introduced cDNA(s)

  • RAFL05-03-O18 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-03-O18 - Resource: pda00856

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-03-O18 - full reading 
    GCTTTTCAAT CTTCGTCTAT CTGTCTCCAC GATCCACGCC TTGACCTTCG 
    TTTTTTTTTT CTCAGAGATT AGAGAAAACT CCGATAACCA ATTTCTCAAT 
    CTTTTTGTAG ATCCAATTTT TCCAGGAAAA AAAGAGGTTT CGCGAAGAAG 
    ATGAGTTTTC AAGATTTAGA ATCAGGAAGA GGAAGATCAA CGAGGAAATT 
    CAATGGTGGT CGACAAGATT CGACACAAGC CGTTGCTTCT GGAATATTCC 
    AGATCAATAC CGGAGTCTCC ACGTTTCAGC GACTCGTTAA CACACTTGGT 
    ACTCCCAAAG ATACACCGGA GCTCCGGGAG AAGCTGCACA AGACAAGGTT 
    GCATATTGGA CAGCTTGTGA AGGATACTTC AGCTAAACTT AAAGAAGCTA 
    GTGAAACTGA TCATCAAAGC GGTGTCAATC CAAGTAAGAA GATTGCAGAT 
    GCTAAGCTTG CAAGGGACTT TCAAGCTGTT TTAAAAGAGT TTCAGAAAGC 
    TCAACAAACT GCTGCTGAAA GAGAAACCAC TTATACTCCT TTCGTGCCTC 
    AATCTGCTCT TCCCTCTAGC TATACGGCGG GTGAGGTGGA TAAGGTACCG 
    GAACAGCGGG CACAACTTCA GGAGTCGAAA AGACAAGAAC TTGTATTGTT 
    AGACAACGAG ATTGCATTCA ACGAGGCTGT GATTGAAGAA AGAGAGCAAG 
    GAATACAAGA AATCCATCAG CAAATTGGTG AGGTTAACGA GATATTCAAA 
    GATCTTGCGG TTCTGGTTAA CGATCAAGGA GTCATGATAG ATGATATTGG 
    TACTCACATT GACAACTCCC GTGCTGCAAC TTCACAAGGA AAATCTCAGC 
    TTGTGCAAGC CGCGAAAACA CAAAAATCAA ACTCCTCTCT GACATGCTTG 
    CTCTTGGTGA TATTTGGCAT TGTTCTCCTG ATCGTGATTA TAGTACTCGC 
    AGCTTGATTT CGAACAGCAA GAACATTTCG TTTGTGTGTG TTCATTGATC 
    TTAAGGCATC AATGACTGGA ACTACTTGGT ATATATTATA TGTTGAAGAG 
    AACACTTAGA AGTTGGTTTT GTGTTTGTTT GGGTTGGTTC AGTTCGGTTT 
    GAGGTTCTAT TGGTTAATGT AATAGTGTGT GCGTGTGTGG CTTTTTTGTT 
    GTGCAAACAT TTTGTTGGAG ATGACAGAAA CTCTGTTTTG TTTGTGATTC 
    TCCTATGTTC TCCTAAATCT TTGTTGAGAG GAAACAAAAA CTATTTGCTC 
    G

    Gene models with high sequence identity

    1. AT5G46860.1 [+] show detail - Syntaxin/t-SNARE family protein
      E-value: 0; Score: 100.00

    AT5G46860.1

    Model type
    Protein coding
    Short Description
    Syntaxin/t-SNARE family protein
    Curator Summary
    Syntaxin-related protein required for vacuolar assembly. A member of t-SNARE superfamily, homologous to yest Vam3p. Localized in the vacuolar membranes. The protein has a heptad repeat structure (residues164
    Computational Description
    VACUOLAR MORPHOLOGY 3 (VAM3); FUNCTIONS IN: SNAP receptor activity; INVOLVED IN: in 7 processes; LOCATED IN: late endosome, trans-Golgi network transport vesicle, plant-type vacuole membrane, vacuole, plant-type vacuole; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Target SNARE coiled-coil domain (InterPro:IPR000727), t-SNARE (InterPro:IPR010989), Syntaxin/epimorphin, conserved site (InterPro:IPR006012), Syntaxin, N-terminal (InterPro:IPR006011); BEST Arabidopsis thaliana protein match is: syntaxin of plants 23 (TAIR:AT4G17730.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR