F28935

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28935

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28935

Determination of introduced cDNA(s)

  • RAFL09-54-N06 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-54-N06 - Resource: pda17759

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-54-N06 - full reading 
    GAACAAACCA ACTTCAGCGA CAAGATAACA AAACGCTTTC ACACGATCGG 
    ATAAAATGGC GGAGAAGAGC TTTAAGTACA TCATCCTCGG CGGCGGCGTC 
    TCTGCCGGAT ACGCAGCTAA GGAGTTTGCC AATCAAGGGG TTCAACCAGG 
    AGAATTGGCT GTTATCTCGA AAGAGGCGGT GGCTCCATAT GAACGCCCTG 
    CACTTAGCAA GGGGTATTTG TTTCCTGAAG GGGCTGCTAG ACTTCCAGGT 
    TTCCATTGCT GTGTTGGTAG TGGTGGAGAA AAACTGCTTC CTGAATCCTA 
    CAAGCAGAAA GGAATTGAGT TGATACTAAG CACAGAAATA GTGAAAGCTG 
    ATCTCTCTGC CAAGAGTCTT GTCAGTGCAA CTGGGGATGT CTTCAAGTAT 
    CAGACGCTCA TAATTGCAAC TGGTTCTACT GTTCTCAGAT TGACTGATTT 
    TGGAGTAAAA GGTGCAGACT CTAAGAATAT CCTCTATCTG AGAGAGATTG 
    ATGATGCCGA CAAATTGGTT GAAGCAATTA AAGCTAAGAA AGGTGGAAAG 
    GCTGTAGTTG TTGGCGGAGG TTACATTGGT CTTGAGCTTA GTGCAGTTTT 
    AAGGATCAAC AATTTAGATG TCACTATGGT TTTCCCTGAA CCTTGGTGCA 
    TGCCTAGGCT TTTCACCGCC GATATTGCTG CTTTCTATGA GACTTACTAT 
    ACAAACAAGG GAGTGAAGAT CATTAAAGGA ACAGTGGCAT CTGGGTTCAC 
    AGCTCAGCCA AATGGAGAGG TGAAGGAAGT ACAACTCAAG GATGGGAGGA 
    CATTGGAAGC CGACATTGTG ATAGTTGGGG TTGGTGCAAA ACCATTGACA 
    TCCTTATTCA AGGGACAGGT CGAGGAAGAT AAAGGTGGAA TTAAGACCGA 
    TGCATTCTTC AAAACAAGTG TTCCAGATGT TTACGCTGTT GGTGACGTTG 
    CTACTTTCCC TTTGAAAATG TACGGAGACG TGAGAAGGGT CGAGCATGTT 
    GACCATTCCC GCAAATCAGC AGAGCAAGCT GTTAAGGCGA TCAAAGCGGC 
    TGAGGGAGGC GCAGCGGTGG AGGAATACGA CTATCTTCCA TTCTTCTACT 
    CTCGCTCCTT TGATCTCTCA TGGCAGTTCT ACGGAGACAA CGTAGGAGAC 
    TCTGTGTTAT TTGGAGACAG CAATCCATCA AACCCAAAAC CAAGATTTGG 
    AGCATATTGG GTTCAAGGTG GTAAAGTGGT TGGAGCATTC ATGGAGGGAG 
    GTAGTGGTGA TGAGAACAAA GCCTTGGCTA AAGTCGCCAA AGCTCGACCT 
    TCTGCAGAGA GTCTCGACGA GCTCGTGAAA CAAGGAATTT CCTTCGCAGC 
    TAAGATCTGA GTGAAACACA ACCACCAAAA CAAACTCACG TTTTCTCTTC 
    TATGTTAAAA TAAAAACTTG TTTAGCTATA ATAAGTTGTG TGTTTGTATT 
    GTCTCTCTAA ACTCCATGAG AAGAGTCTGG CAGAGGAAAC CCTGAACCAC 
    ATTCACATTT TAGATCATGT GTTTGGTTTT CTGTGTTCCA AAAAAAAAA

    Gene models with high sequence identity

    1. AT3G52880.1 [+] show detail - monodehydroascorbate reductase 1
      E-value: 0; Score: 99.87

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.5e-33
    HMMPfam Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region 8.9e-16
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 3.1e-22
    FPrintScan PNDRDTASEI 3.5e-17
    FPrintScan PNDRDTASEI 3.5e-17
    FPrintScan PNDRDTASEI 3.5e-17
    FPrintScan PNDRDTASEI 3.5e-17
    Gene3D no description 1.9e-14
    Gene3D no description 1.5e-36
    superfamily FAD/NAD(P)-binding domain 4.9e-38
    superfamily FAD/NAD-linked reductase, dimerisation 2.2e-17
    HMMPanther MONODEHYDROASCORBATE REDUCTASE (NADH) 6.4e-188
    HMMPanther DISULFIDE OXIDOREDUCTASE 6.4e-188

    See the detailed result >

    AT3G52880.1

    Model type
    Protein coding
    Short Description
    monodehydroascorbate reductase 1
    Curator Summary
    Encodes a peroxisomal monodehydroascorbate reductase, involved in the ascorbate-glutathione cycle which removes toxic H2O2
    Computational Description
    monodehydroascorbate reductase 1 (MDAR1); FUNCTIONS IN: monodehydroascorbate reductase (NADH) activity; INVOLVED IN: response to cadmium ion, peptidyl-cysteine S-nitrosylation, hydrogen peroxide catabolic process; LOCATED IN: peroxisomal matrix, apoplast, chloroplast, peroxisome, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: FAD-dependent pyridine nucleotide-disulphide oxidoreductase (InterPro:IPR013027), FAD/NAD-linked reductase, dimerisation (InterPro:IPR016156), Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region (InterPro:IPR001327); BEST Arabidopsis thaliana protein match is: Pyridine nucleotide-disulphide oxidoreductase family protein (TAIR:AT5G03630.1); Has 31553 Blast hits to 31492 proteins in 3048 species: Archae - 709; Bacteria - 24685; Metazoa - 865; Fungi - 647; Plants - 730; Viruses - 0; Other Eukaryotes - 3917 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR