F29519

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F29519

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F29519

Determination of introduced cDNA(s)

  • RAFL07-10-I24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL07-10-I24 - Resource: pda04091

    full reading [+] show sequence

    Nucleotide sequence: RAFL07-10-I24 - full reading 
    GCTCGCATCT TCTTCTTCTT CACGGAGAAG CAATCGAAGA GAATCAATCA 
    ACTAGACAAA AATCGGAAGA AGAAACACAA ACCGAGGTTT TAGAAACATG 
    GCGTCTAAGG TAATCTCTGC CACAATCCGC AGAACCCTAA CCAAACCACA 
    CGGCACTTTT TCCCGGTGTC GCTACTTATC AACCGCCGCT GCTGCGACGG 
    AGGTGAATTA CGAGGATGAA TCGATTATGA TGAAAGGAGT TCGAATTTCA 
    GGTAGACCTC TTTACTTAGA TATGCAAGCG ACGACTCCGA TTGATCCTAG 
    GGTATTCGAT GCGATGAATG CTTCACAGAT CCATGAGTAT GGGAATCCTC 
    ACTCGCGAAC GCATCTCTAC GGCTGGGAAG CTGAGAACGC CGTCGAGAAC 
    GCACGAAACC AGGTCGCGAA ACTGATCGAA GCTTCACCGA AGGAGATCGT 
    ATTCGTGTCC GGTGCAACGG AGGCGAACAA TATGGCGGTG AAAGGAGTGA 
    TGCACTTTTA CAAGGACACG AAGAAACATG TGATAACTAC ACAGACTGAG 
    CATAAGTGTG TGCTTGATTC GTGTAGGCAT TTGCAGCAAG AAGGATTTGA 
    GGTAACTTAT TTACCTGTGA AAACTGATGG ATTGGTTGAT TTAGAGATGT 
    TGAGAGAAGC TATTAGGCCA GACACAGGGC TAGTTTCTAT TATGGCTGTG 
    AACAATGAGA TTGGTGTGGT TCAACCTATG GAGGAGATTG GTATGATTTG 
    CAAAGAGCAT AATGTTCCGT TTCATACTGA TGCTGCTCAA GCTATTGGGA 
    AGATACCTGT TGATGTTAAG AAGTGGAATG TTGCTTTGAT GTCTATGAGT 
    GCTCACAAGA TCTATGGACC GAAAGGTGTT GGTGCTTTGT ATGTGAGGAG 
    GAGGCCGAGA ATCAGGCTTG AGCCGTTGAT GAATGGTGGA GGTCAGGAGA 
    GGGGATTGCG TAGTGGTACG GGGGCTACGC AGCAGATTGT TGGGTTCGGG 
    GCTGCTTGTG AGTTGGCTAT GAAGGAGATG GAGTATGATG AGAAGTGGAT 
    TAAGGGGTTA CAGGAGAGGT TGCTGAATGG GGTTAGAGAG AAGCTTGATG 
    GTGTTGTGGT GAATGGTTCA ATGGATAGTC GATATGTAGG GAATTTGAAT 
    TTGTCGTTTG CTTATGTTGA AGGAGAGAGT TTGTTGATGG GATTGAAGGA 
    AGTTGCAGTG TCTAGTGGAA GTGCTTGTAC TAGTGCGAGT TTGGAGCCTT 
    CTTATGTGTT GAGAGCTTTG GGTGTGGATG AAGACATGGC TCACACTTCG 
    ATTAGGTTTG GGATTGGTAG GTTTACCACG AAGGAAGAGA TTGATAAAGC 
    GGTCGAGCTT ACGGTTAAAC AAGTTGAGAA GTTGAGGGAA ATGAGCCCGC 
    TTTATGAAAT GGTTAAAGAA GGTATCGATA TCAAGAACAT TCAATGGTCT 
    CAACACTGAT TCAACAGTTC CACTCAAACT GTGCGGGACA AGAGGGAATC 
    TCCAGTCTGT GGTCAGCCGG GGTATATGAG AGCCATACTG AGATGTGAGT 
    ATGAACGATG ATATGATCCT GTGTTCTTAC TATGTTCTGT TGGTAAGTTA 
    TCTATCTATA TTGCTTTCTA GAGAGAATTG CATGTGAGAG TTTCTTGTGT 
    GGTTTTGTAA TAAAACAGAA ACAAAGGTTC ATTTCTGGTT AGATTGTGAT 
    ATCCTTTGAA ATTGAATGAT TAATTTTTAG GTGTTGTGAG CTTAGCTTTT 
    TTAGCTACGG TAATGGTGAA GTTTGTTTTG TTTTTCTTCT TATTTATGAA 
    AATAAAATAC AATTTTATGT GAAAGAAAAA AAAAAAAAAA A

    Gene models with high sequence identity

    1. AT5G65720.1 [+] show detail - nitrogen fixation S (NIFS)-like 1
      E-value: 0; Score: 99.95

     

    InterPro Scan Digest

    Program Description E-value
    HMMPIR Cysteine desulfurase, NifS 1.5e-170
    HMMPfam Aminotransferase, class V/Cysteine desulfurase 1.2e-89
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 1.9e-94
    HMMTigr Cysteine desulfurase 8.5e-229
    HMMPanther CYSTEINE DESULFURYLASE 1.9e-161
    superfamily Pyridoxal phosphate-dependent transferase, major domain 1.5e-114

    See the detailed result >

    AT5G65720.1

    Model type
    Protein coding
    Short Description
    nitrogen fixation S (NIFS)-like 1
    Curator Summary
    cysteine desulfurase whose activity is dependent on AtSufE activation.
    Computational Description
    nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cysteine desulfurase (InterPro:IPR010240), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR