F29519

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F29519

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F29519

Determination of introduced cDNA(s)

  • RAFL07-10-I24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL07-10-I24 - Resource: pda04091

    full reading [+] show sequence

    Nucleotide sequence: RAFL07-10-I24 - full reading
    GCTCGCATCT TCTTCTTCTT CACGGAGAAG CAATCGAAGA GAATCAATCA 
    ACTAGACAAA AATCGGAAGA AGAAACACAA ACCGAGGTTT TAGAAACATG
    GCGTCTAAGG TAATCTCTGC CACAATCCGC AGAACCCTAA CCAAACCACA
    CGGCACTTTT TCCCGGTGTC GCTACTTATC AACCGCCGCT GCTGCGACGG
    AGGTGAATTA CGAGGATGAA TCGATTATGA TGAAAGGAGT TCGAATTTCA
    GGTAGACCTC TTTACTTAGA TATGCAAGCG ACGACTCCGA TTGATCCTAG
    GGTATTCGAT GCGATGAATG CTTCACAGAT CCATGAGTAT GGGAATCCTC
    ACTCGCGAAC GCATCTCTAC GGCTGGGAAG CTGAGAACGC CGTCGAGAAC
    GCACGAAACC AGGTCGCGAA ACTGATCGAA GCTTCACCGA AGGAGATCGT
    ATTCGTGTCC GGTGCAACGG AGGCGAACAA TATGGCGGTG AAAGGAGTGA
    TGCACTTTTA CAAGGACACG AAGAAACATG TGATAACTAC ACAGACTGAG
    CATAAGTGTG TGCTTGATTC GTGTAGGCAT TTGCAGCAAG AAGGATTTGA
    GGTAACTTAT TTACCTGTGA AAACTGATGG ATTGGTTGAT TTAGAGATGT
    TGAGAGAAGC TATTAGGCCA GACACAGGGC TAGTTTCTAT TATGGCTGTG
    AACAATGAGA TTGGTGTGGT TCAACCTATG GAGGAGATTG GTATGATTTG
    CAAAGAGCAT AATGTTCCGT TTCATACTGA TGCTGCTCAA GCTATTGGGA
    AGATACCTGT TGATGTTAAG AAGTGGAATG TTGCTTTGAT GTCTATGAGT
    GCTCACAAGA TCTATGGACC GAAAGGTGTT GGTGCTTTGT ATGTGAGGAG
    GAGGCCGAGA ATCAGGCTTG AGCCGTTGAT GAATGGTGGA GGTCAGGAGA
    GGGGATTGCG TAGTGGTACG GGGGCTACGC AGCAGATTGT TGGGTTCGGG
    GCTGCTTGTG AGTTGGCTAT GAAGGAGATG GAGTATGATG AGAAGTGGAT
    TAAGGGGTTA CAGGAGAGGT TGCTGAATGG GGTTAGAGAG AAGCTTGATG
    GTGTTGTGGT GAATGGTTCA ATGGATAGTC GATATGTAGG GAATTTGAAT
    TTGTCGTTTG CTTATGTTGA AGGAGAGAGT TTGTTGATGG GATTGAAGGA
    AGTTGCAGTG TCTAGTGGAA GTGCTTGTAC TAGTGCGAGT TTGGAGCCTT
    CTTATGTGTT GAGAGCTTTG GGTGTGGATG AAGACATGGC TCACACTTCG
    ATTAGGTTTG GGATTGGTAG GTTTACCACG AAGGAAGAGA TTGATAAAGC
    GGTCGAGCTT ACGGTTAAAC AAGTTGAGAA GTTGAGGGAA ATGAGCCCGC
    TTTATGAAAT GGTTAAAGAA GGTATCGATA TCAAGAACAT TCAATGGTCT
    CAACACTGAT TCAACAGTTC CACTCAAACT GTGCGGGACA AGAGGGAATC
    TCCAGTCTGT GGTCAGCCGG GGTATATGAG AGCCATACTG AGATGTGAGT
    ATGAACGATG ATATGATCCT GTGTTCTTAC TATGTTCTGT TGGTAAGTTA
    TCTATCTATA TTGCTTTCTA GAGAGAATTG CATGTGAGAG TTTCTTGTGT
    GGTTTTGTAA TAAAACAGAA ACAAAGGTTC ATTTCTGGTT AGATTGTGAT
    ATCCTTTGAA ATTGAATGAT TAATTTTTAG GTGTTGTGAG CTTAGCTTTT
    TTAGCTACGG TAATGGTGAA GTTTGTTTTG TTTTTCTTCT TATTTATGAA
    AATAAAATAC AATTTTATGT GAAAGAAAAA AAAAAAAAAA A

    Gene models with high sequence identity

    1. AT5G65720.1 [+] show detail - nitrogen fixation S (NIFS)-like 1
      E-value: 0; Score: 99.95

     

    InterPro Scan Digest

    Program Description E-value
    HMMPIR Cysteine desulfurase, NifS 1.5e-170
    HMMPfam Aminotransferase, class V/Cysteine desulfurase 1.2e-89
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 1.9e-94
    HMMTigr Cysteine desulfurase 8.5e-229
    HMMPanther CYSTEINE DESULFURYLASE 1.9e-161
    superfamily Pyridoxal phosphate-dependent transferase, major domain 1.5e-114

    See the detailed result >

    AT5G65720.1

    Model type
    Protein coding
    Short Description
    nitrogen fixation S (NIFS)-like 1
    Curator Summary
    cysteine desulfurase whose activity is dependent on AtSufE activation.
    Computational Description
    nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cysteine desulfurase (InterPro:IPR010240), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR