F29732

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F29732

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F29732

Determination of introduced cDNA(s)

  • RAFL05-02-M11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-02-M11 - Resource: pda00713

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-02-M11 - full reading 
    AAAATTTTCC CCATTAAACA AAAAAAAATC AAATCTCTCT CTTTCTCTCT 
    CTAATGGCGG CGACATTAGG CAGAGACCAG TATGTGTACA TGGCGAAGCT 
    CGCCGAGCAG GCGGAGCGTT ACGAAGAGAT GGTTCAATTC ATGGAACAGC 
    TCGTTACAGG CGCTACTCCA GCGGAAGAGC TCACCGTTGA AGAGAGGAAT 
    CTCCTCTCTG TTGCTTACAA AAACGTGATC GGATCTCTAC GCGCCGCCTG 
    GAGGATCGTG TCTTCGATTG AGCAGAAGGA AGAGAGTAGG AAGAACGACG 
    AGCACGTGTC GCTTGTCAAG GATTAAAGAT CTAAAGTTGA GTCTGAGCTT 
    TCTTCTGTTT GCTCTGGAAT CCTTAAGCTC CTTGACTCGC ATCTGATCCC 
    ATCTGCTGGA GCGAGTGAGT CTAAGGTCTT TTACTTGAAG ATGAAAGGTG 
    ATTATCATCG GTACATGGCT GAGTTTAAGT CTGGTGATGA GAGGAAAACT 
    GCTGCTGAAG ATACCATGCT CGCTTACAAA GCAGCTCAGG ATATCGCAGC 
    TGCGGATATG GCACCTACTC ATCCGATAAG GCTTGGTCTG GCCCTGAATT 
    TCTCAGTGTT CTACTATGAG ATTCTCAATT CTTCAGACAA AGCTTGTAAC 
    ATGGCCAAAC AGGCTTTTGA GGAGGCCATA GCTGAGCTTG ACACTCTGGG 
    AGAGGAATCC TACAAAGACA GCACTCTCAT AATGCAGTTG CTGAGGGACA 
    ATTTAACCCT TTGGACCTCC GATATGCAGG AGCAGATGGA CGAGGCCTGA 
    GGATCTAGAT GAAGGGGGGG AGGGTTGTTA CGCGATGTTT CTGCCACCAA 
    ATCGATCTCA AAATCCCCAT AACCTTTGCT CAAAAACTGT GAAAAAAGAT 
    TGAAGTGTTT ATGATGATTA TGATTGTGCA CAGCTTGATG ATTTATCTAC 
    TCTACTAAAC CTCTGTGCTC TTAATATTTA TTGTCTCGAC TCTGCTCAAG 
    CCTTAAAAAC ATCTTTCTCC TTAAGGCTCC TCTGGTTAAA TTATGATCCT 
    TCTACTGTAT CGTCAAAATC AAGTTGGAAC CCAAAATTCG TGGATT

    Gene models with high sequence identity

    1. AT5G10450.1 [+] show detail - G-box regulating factor 6
      E-value: 0; Score: 99.91

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart 14-3-3 protein 0.00012
    HMMSmart 14-3-3 protein 6.8e-82
    PatternScan 14-3-3 protein 0
    PatternScan 14-3-3 protein 0
    HMMPfam 14-3-3 protein 9.8e-31
    HMMPfam 14-3-3 protein 4.9e-74
    FPrintScan 14-3-3 protein 2.6e-58
    FPrintScan 14-3-3 protein 2.6e-58
    FPrintScan 14-3-3 protein 2.6e-58
    FPrintScan 14-3-3 protein 2.6e-58
    superfamily 14-3-3 protein 1.4e-26
    superfamily 14-3-3 protein 9.4e-68
    HMMPanther 14-3-3 protein 5e-53
    HMMPanther 14-3-3 protein 6.6e-123
    Coil coiled-coil 0
    Coil coiled-coil 0
    Gene3D 14-3-3 protein 1.2e-23
    Gene3D 14-3-3 protein 3.7e-68

    See the detailed result >

    AT5G10450.1

    Model type
    Protein coding
    Short Description
    G-box regulating factor 6
    Curator Summary
    Encodes a member of the 14-3-3 gene family that is a lambda isoform (14-3-3λ). Interacts with APX3 (ascorbate peroxidase) and AKR2 , suggesting a role in mediating oxidative metabolism in stress response. This protein was shown to colocalize and interact with SERK1 by which it is phosphorylated. This protein is also reported to interact with the phosphorylated form of the BZR1 transcription factor involved in brassinosteroid signaling and may affect the nucleocytoplasmic shuttling of BZR1.
    Computational Description
    G-box regulating factor 6 (GRF6); FUNCTIONS IN: protein phosphorylated amino acid binding; INVOLVED IN: brassinosteroid mediated signaling pathway, response to cadmium ion, defense response to bacterium, phosphorylation; LOCATED IN: in 6 components; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: 14-3-3 protein (InterPro:IPR000308); BEST Arabidopsis thaliana protein match is: general regulatory factor 8 (TAIR:AT5G65430.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR