F29911

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) decreased size (PATO:0000587) (Rosette leaf::Shape::small) abnormal (PATO:0000460) (Rosette leaf::Others) present in fewer numbers in organism (PATO:0001997) (Rosette leaf::Number::few)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F29911

Determination of introduced cDNA(s)

RAFL05-16-I21 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-16-I21 - Resource: pda02689

full reading [+] show sequence

GCCTTTGTCT CTCATTCTTC TTCTCCATTT CGAAAAGAAG ATGAAGGTGA 
TAGATAAGAT CCAGTCATTA GCCGACGAAG GCAAAACAGC GTTTTCCTTC 
GAATTCTTCC CACCAAAGAC AGAAGATGGA GTCGACAATC TCTTTGAGAG 
AATGGATCGA ATGGTTGCTT ATGGTCCAAC GTTTTGCGAT ATCACTTGGG 
GAGCTGGTGG ATCTACCGCA GATCTAACGC TTGATATAGC TTCGAGGATG 
CAAAACGTTG TTTGTGTTGA GAGTATGATG CATCTTACGT GTACTAACAT 
GCCTGTTGAG AAGATTGATC ATGCTCTTGA GACTATTAGA TCTAATGGGA 
TTCAGAATGT GCTTGCTCTT AGAGGTGATC CTCCTCATGG TCAAGATAAG 
TTTGTTCAGG TTGAAGGTGG TTTTGATTGT GCTTTGGATC TTGTTAATCA 
CATTCGTAGC AAGTATGGTG ATTACTTTGG TATCACTGTC GCTGGTTATC 
CTGAGGCGCA TCCTGATGTT ATTGGTGAGA ATGGTCTTGC GTCTAATGAA 
GCTTATCAGA GTGATCTTGA GTATCTGAAG AAAAAGATTG ATGCTGGAGC 
TGATCTGATT GTTACTCAGC TTTTCTATGA TACTGATATA TTCCTCAAGT 
TTGTGAATGA TTGTCGGCAA ATTGGGATTA GTTGTCCCAT TGTTCCTGGA 
ATTATGCCTA TTAATAACTA CAGAGGCTTT TTGCGTATGA CTGGTTTCTG 
CAAGACTAAG ATACCAGTTG AGGTGATGGC TGCCTTGGAG CCTATCAAGG 
ATAACGAAGA AGCTGTGAAA GCCTATGGTA TTCACCTTGG AACAGAGATG 
TGTAAAAAGA TGTTGGCTCA TGGAGTCAAG TCTCTTCATC TCTACACATT 
GAACATGGAG AAATCTGCTC TTGCAATATT GATGAATCTT GGTATGATTG 
ATGAGTCCAA AATTTCTCGT TCTTTACCCT GGAGACGCCC TGCAAATGTT 
TTCCGTACTA AGGAAGATGT GCGCCCCATT TTCTGGGCAA ACCGTCCAAA 
GAGCTACATT TCTAGAACCA AGGGCTGGGA AGATTTCCCA CAAGGCCGGT 
GGGGTGATTC ACGCAGTGCT TCATATGGTG CACTCTCGGA TCATCAGTTC 
TCACGTCCGC GAGCACGTGA CAAGAAGCTT CAACAAGAAT GGGTTGTCCC 
ACTGAAAAGT GTTGAAGATA TTCAGGAGAA ATTCAAGGAG CTCTGCCTTG 
GAAACCTTAA AAGCAGTCCC TGGTCTGAAT TAGATGGACT CCAGCCAGAG 
ACAAGGATAA TAAACGAGCA ACTGATAAAA GTCAACTCCA AAGGCTTCTT 
GACCATCAAT AGCCAACCAT CAGTCAACGC CGAGAGATCT GATTCCCCAA 
CTGTTGGATG GGGAGGTCCT GTTGGATATG TATACCAAAA GGCTTATCTA 
GAGTTCTTCT GCTCAAAGGA GAAATTAGAT GCAGTGGTGG AGAAATGCAA 
AGCTTTGCCA TCGATTACTT ACATGGCTGT GAACAAAGGA GAACAGTGGG 
TATCGAACAC TGCCCAAGCT GATGTGAATG CTGTAACTTG GGGAGTTTTC 
CCGGCTAAGG AAATCATTCA ACCAACCATT GTCGATCCCG CTAGCTTCAA 
CGTCTGGAAG GATGAAGCAT TTGAGACTTG GTCAAGAAGC TGGGCTAACT 
TGTACCCAGA AGCCGACCCT TCCAGAAACT TGCTCGAGGA GGTGAAGAAC 
AGCTACTATT TGGTAAGCTT GGTGGAGAAC GATTACATCA ATGGTGACAT 
ATTCGCGGTG TTTGCTGATC TTTGAGAAAA TCAAGAGACA ACTACAGTAT 
ATCGGTTTCC TACTGCTTTC AAGATTTGCT TTTGTACTTT CTCTAAAATA 
TTGGGATTTT GTTTTCT

Gene models with high sequence identity

AT2G44160.1 [+] show detail - methylenetetrahydrofolate reductase 2
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMTigr	Eukaryotic-type methylenetetrahydrofolate reductase	2e-238
Gene3D	no description	3.3e-97
HMMPfam	Methylenetetrahydrofolate reductase	4.6e-127
superfamily	FAD-linked oxidoreductase	1.4e-95
HMMPanther	METHYLENETETRAHYDROFOLATE REDUCTASE	2.6e-215
HMMPanther	METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRA	2.6e-215

See the detailed result >

AT2G44160.1

Model type: Protein coding
Short Description: methylenetetrahydrofolate reductase 2
Curator Summary: methylenetetrahydrofolate reductase MTHFR2 mRNA, complete
Computational Description: methylenetetrahydrofolate reductase 2 (MTHFR2); FUNCTIONS IN: methylenetetrahydrofolate reductase (NADPH) activity; INVOLVED IN: oxidation reduction, methionine metabolic process; EXPRESSED IN: male gametophyte, guard cell, cultured cell, leaf; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Methylenetetrahydrofolate reductase (InterPro:IPR003171), Eukaryotic-type methylenetetrahydrofolate reductase (InterPro:IPR004621); BEST Arabidopsis thaliana protein match is: methylenetetrahydrofolate reductase 1 (TAIR:AT3G59970.3); Has 4605 Blast hits to 4574 proteins in 1845 species: Archae - 16; Bacteria - 3444; Metazoa - 135; Fungi - 300; Plants - 75; Viruses - 0; Other Eukaryotes - 635 (source: NCBI BLink).
Link: InterPro Scan - TAIR